Protein A/G-Biotin
-
Catalog number6506-1
-
PricePlease ask
-
Size1 mg
-
-
SynonymsProtein A/G
-
Alternative_namesProtein A/G
-
DescriptionGenetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
-
RecombinantYes
-
SourceE. coli
-
Purity by SDS PAGE≥98%
-
AssaySDS-PAGE
-
Purity≥98%
-
Binding CapacityUnder optimal conditions, 1 mg protein A/G will bind approximately 5 mg human IgG. Optimal binding of Protein A/G to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
-
Molecular Weight59.7 kDa
-
Storage Temp-20°C
-
ShippingGel Pack
-
Shelf Life12 months
-
AppearanceLyophilized
-
Physical form descriptionLyophilized with no additives
-
Reconstitution InstructionsReconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
-
Background InformationProtein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. Protein A/G-Biotin is prepared by conjugating recombinant Protein A/G to Biotin. The conjugation was optimized to provide the maximal binding capacity for IgG.
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
ConjugationBiotinylated
-
Gene target
-
Gene symbolGPRC6A, GPR162, PURG
-
Short nameProtein A/G-
-
LabelBiotin
-
Alternative nameProtein A/G-biotinilated
-
Gene info
-
Identity
-
Gene
-
Long gene nameG protein-coupled receptor class C group 6 member A
-
Synonyms gene name
- G protein-coupled receptor, family C, group 6, member A
- G protein-coupled receptor, class C, group 6, member A
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2002-04-26
-
Entrez gene record
-
Classification
- Calcium sensing receptors
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameG protein-coupled receptor 162
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2005-05-09
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- G protein-coupled receptors, Class A orphans
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namepurine rich element binding protein G
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2004-02-09
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID