PicoProbe™ Phosphoglucomutase Assay Kit (Fluorometric)

• Catalog number
  K770-100
• Price
  Please ask
• Size
  100 assays

• Description
  The most sensitive kit on the market
• Summary
  • Detection method- Fluorescence (Ex/Em = 535/587 nm) • Application- Measurement of phosphoglucomutase activity in various tissues/cells. • Analysis of glucose metabolism and cell signaling in various cell types. • Screening of anti-diabetic drugs.
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm)
• Applications
  • Measurement of phosphoglucomutase activity in various tissues/cells. • Analysis of glucose metabolism and cell signaling in various cell types. • Screening of anti-diabetic drugs
• Sample Type
  • Animal tissues: muscle, liver, heart, kidney etc. • Cell culture: adherent or suspension cells. • Plasma.
• Features Benefits
  • Rapid, Convenient and Reliable • The assay can detect as low as 20 μU of PGM activity
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Phosphoglucomutase (PGM) enzyme plays a key role in carbohydrate metabolism and widely exists in all organisms. PGM interconverts Glucose-1-Phosphate (G1P) and Glucose-6-Phosphate (G6P) depending on the body requirement. When glycogen breaks down, G1P is generated and phosphoglucomutase converts G1P to G6P, which can go either to glycolytic pathway to generate ATP, or to pentose phosphate pathway to generate ribose and NADPH. On the other hand, when cells have extra energy, PGM converts G6P to G1P, which generates glycogen. In humans, phosphoglucomutase has 2 isoforms (PGM I and PGM II). PGM deficiency leads to glucose storage disease. Detection of abnormal phosphoglucomutase activity is crucial for diagnosis, prediction and treatment of the disease. In BioVision’s phosphoglucomutase assay, PGM converts glucose-1-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADPH, which reduces PicoProbe™ to generate an intense fluorescence product (Ex/Em = 535/587 nm). This assay kit is simple, highly sensitive, and rapid and can detect as low as 20 μU of PGM activity.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Conjugate
  Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit

• Gene target
  Phosphoglucomutase   Kit   Fluorometric  
• Short name
  ™ Phosphoglucomutase Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  PicoProbe™ Phosphoglucomutase test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products