Phospho-C/EBP β (Thr235) Antibody

• Catalog number
  A1253-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  CEBPB
• Target Alternative Name
  CCAAT/enhancer-binding protein beta, C/EBP beta, LAP, Liver activator protein, Liver-enriched inhibitory protein, LIP, Nuclear factor NF-IL6, Transcription factor 5, TCF-5
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, Mouse, Rat
• Immunogen Sequence
  Synthesized peptide derived from human C/EBP β around the phosphorylation site of T235. AA: 180-260
• Accession
  P17676
• Gene Id
  1051
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
• Purification
  Affinity-chromatography using epitope-specific immunogen
• Application
  WB, ELISA, IHC, IF
• Positive Control
  WB: HepG2 cell lysate
• Application And Usages
  WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000
• Description
  Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (By similarity). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation.
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Phospho-C/EBP   Thr235  
• Gene symbol
  GLB1, EBP, CEBPG, CEBPA, FCN2, SH3D19, CEBPB, CEBPD
• Short name
  Phospho-C/EBP β (Thr235) Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  phosphorilated-C/EBP β (Thr235) (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:4298
• Gene
  GLB1
• Long gene name
  galactosidase beta 1
• Synonyms gene
  • ELNR1
• Synonyms gene name
  • elastin receptor 1, 67kDa
  • elastin receptor 1 (67kD)
  • galactosidase, beta 1
• Synonyms
  • EBP
• Synonyms name
  • elastin binding protein
• GenBank acession
  • M22590
• Locus
  3p22.3
• Discovery year
  2001-06-22
• Entrez gene record
  2720
• Pubmed identfication
  • 110522
  • 3143362
• RefSeq identity
  • NM_000404
• Classification
  • Galactosidases beta
• VEGA ID
  OTTHUMG00000155781

Gene info

• Identity
  HGNC:3133
• Gene
  EBP
• Long gene name
  EBP cholestenol delta-isomerase
• Synonyms gene
  • CDPX2
• Synonyms gene name
  • emopamil-binding protein (sterol isomerase)
  • emopamil binding protein (sterol isomerase)
• Synonyms
  • CPX
  • CPXD
  • CHO2
• Synonyms name
  • 3-beta-hydroxysteroid-delta-8,delta-7-isomerase
  • Chondrodysplasia punctata-2, X-linked dominant (Happle syndrome)
  • sterol 8-isomerase
• GenBank acession
  • Z37986
• Locus
  Xp11.23
• Discovery year
  2000-02-21
• Entrez gene record
  10682
• Pubmed identfication
  • 7706302
  • 8938429
• RefSeq identity
  • NM_006579
• VEGA ID
  OTTHUMG00000034482

Gene info

• Identity
  HGNC:1837
• Gene
  CEBPG
• Long gene name
  CCAAT enhancer binding protein gamma
• Synonyms gene name
  • CCAAT/enhancer binding protein (C/EBP), gamma
  • CCAAT/enhancer binding protein gamma
• Synonyms
  • GPE1BP
  • IG/EBP-1
• GenBank acession
  • U20240
• Locus
  19q13.11
• Discovery year
  1992-07-08
• Entrez gene record
  1054
• Pubmed identfication
  • 1884998
• RefSeq identity
  • NM_001806
• Classification
  • Basic leucine zipper proteins
  • CCAAT/enhancer binding proteins
• VEGA ID
  OTTHUMG00000180469

Gene info

• Identity
  HGNC:1833
• Gene
  CEBPA
• Long gene name
  CCAAT enhancer binding protein alpha
• Synonyms gene
  • CEBP
• Synonyms gene name
  • CCAAT/enhancer binding protein (C/EBP), alpha
  • CCAAT/enhancer binding protein alpha
• Synonyms
  • C/EBP-alpha
• GenBank acession
  • M37197
• Locus
  19q13.11
• Discovery year
  1991-11-27
• Entrez gene record
  1050
• Pubmed identfication
  • 1535333
  • 1840554
• RefSeq identity
  • NM_004364
• Classification
  • Basic leucine zipper proteins
  • CCAAT/enhancer binding proteins
• VEGA ID
  OTTHUMG00000161461
• Locus Specific Databases
  • LRG_456

Gene info

• Identity
  HGNC:3624
• Gene
  FCN2
• Long gene name
  ficolin 2
• Synonyms gene name
  • ficolin (collagen/fibrinogen domain-containing lectin) 2 (hucolin)
  • ficolin (collagen/fibrinogen domain containing lectin) 2
• Synonyms
  • P35
  • FCNL
  • EBP-37
  • ficolin-2
• Synonyms name
  • hucolin
  • collagen/fibrinogen domain-containing protein 2
  • ficolin B
  • serum lectin p35
  • L-ficolin
• GenBank acession
  • D49353
• Locus
  9q34.3
• Discovery year
  1996-07-11
• Entrez gene record
  2220
• Pubmed identfication
  • 8884275
• RefSeq identity
  • NM_004108
• Classification
  • Complement system activation components
  • Fibrinogen C domain containing
• VEGA ID
  OTTHUMG00000020892

Gene info

• Identity
  HGNC:30418
• Gene
  SH3D19
• Long gene name
  SH3 domain containing 19
• Synonyms
  • DKFZp434D0215
  • EVE1
  • EBP
  • Kryn
  • SH3P19
  • Eve-1
• Synonyms name
  • EEN binding protein
• GenBank acession
  • BX647422
• Locus
  4q31.3
• Discovery year
  2008-02-18
• Entrez gene record
  152503
• Pubmed identfication
  • 12615363
• RefSeq identity
  • NM_001009555
• VEGA ID
  OTTHUMG00000154051

Gene info

• Identity
  HGNC:1834
• Gene
  CEBPB
• Long gene name
  CCAAT enhancer binding protein beta
• Synonyms gene
  • TCF5
• Synonyms gene name
  • CCAAT/enhancer binding protein (C/EBP), beta
  • CCAAT/enhancer binding protein beta
• Synonyms
  • LAP
  • CRP2
  • NFIL6
  • IL6DBP
  • C/EBP-beta
• Synonyms name
  • liver-enriched transcriptional activator protein
  • nuclear factor of interleukin 6
  • interleukin 6-dependent DNA-binding protein
• GenBank acession
  • AY193834
• Locus
  20q13.13
• Discovery year
  1991-02-27
• Entrez gene record
  1051
• Pubmed identfication
  • 1535333
  • 1840554
• RefSeq identity
  • NM_005194
• Classification
  • Basic leucine zipper proteins
  • CCAAT/enhancer binding proteins
• VEGA ID
  OTTHUMG00000032715

Gene info

• Identity
  HGNC:1835
• Gene
  CEBPD
• Long gene name
  CCAAT enhancer binding protein delta
• Synonyms gene name
  • CCAAT/enhancer binding protein (C/EBP), delta
  • CCAAT/enhancer binding protein delta
• Synonyms
  • CRP3
  • CELF
  • C/EBP-delta
  • NF-IL6-beta
• Locus
  8q11.21
• Discovery year
  1992-06-24
• Entrez gene record
  1052
• Pubmed identfication
  • 1840554
  • 1884998
• RefSeq identity
  • NM_005195
• Classification
  • Basic leucine zipper proteins
  • CCAAT/enhancer binding proteins
• VEGA ID
  OTTHUMG00000162290

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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