Phospho-ASK 1 (Ser83) Antibody
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Catalog number
A1251-100
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Price
Please ask
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Size
100 µl
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Antibody Target
MAP3K5
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Target Alternative Name
Apoptosis signal-regulating kinase 1, Mitogen-activated protein kinase kinase kinase 5, ASK-1, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5
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Antibody Type
Polyclonal
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Species Reactivities
Human
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Immunogen Sequence
Synthesized peptide derived from human ASK 1 around the phosphorylation site of S83. AA range: 20-100
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Accession
Q99683
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Gene Id
4217
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
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Purification
Affinity-chromatography using epitope-specific immunogen
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Application
WB, ELISA, IHC
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Positive Control
WB: 293 cell lysate
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Application And Usages
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;
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Description
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans
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Handling
The antibody solution should be gently mixed before use
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
DBF4
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Short name
Phospho-ASK 1 (Ser83) Antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
phosphorilated-ASK 1 (Ser83) (antibody to-)
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
DBF4 zinc finger
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Synonyms gene name
- DBF4 homolog (S. cerevisiae)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2005-11-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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