Peroxiredoxin V Antibody (3F11)
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Catalog number6162-100
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Price:
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Size100 μl
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Antibody TargetPeroxiredoxin V
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Target Alternative NamePeroxiredoxin 5, ACR1, AOEB116, B116, PLP, PMP20, PRDX6, PRXV, SBBI10.
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Antibody TypeMonoclonal
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Clone3F11
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Species ReactivitiesHuman
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Immunogen SequenceRecombinant human Prx V fragment protein (without mitochondrial leader sequence) purified from E.coli.
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AccessionP30044
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Gene Id25824
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AppearanceColorless liquid
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FormLiquid
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Concentration1 mg/ml
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Formulation100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
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PurificationAmmonium sulphate precipitation purified antibody
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ApplicationWestern blot,IP,ICC,ELISA.
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Positive ControlIP analysis : HeLa cell lysate. IHC staining : HeLa cells
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Application And UsagesWestern blot: 1:1000, IP: 1-2 µl, ICC: 20 µl/ml, ELISA.
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DescriptionPeroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx V is involved in reduction of hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short namePeroxiredoxin V Antibody (3F11)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeMouse IgG 1
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Alternative namePeroxiredoxin V (antibody to-) (3F11)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data