PCR-Salmonella-Listeria Detection Kit

• Catalog number
  K1448-96
• Price
  Please ask
• Size
  96 Rxns

• Description
  PCR based Salmonella spp & Listeria monocytogenes Detection Kit
• Summary
  PCR-Salmonella-Listeria Detection Kit is based on is based on amplification and detection of specific DNA fragments from Salmonella spp and/or Listeria monocytogenes by the real-time PCR method (multiplex PCR).
• Detection Method
  Real-time PCR
• Species Reactivity
  Listeria monocytogenes, Salmonella
• Applications
  Real-time PCR
• Sample Type
  Food samples, Bacteria, Salmonella spp & Listeria monocytogenes, Beef, milk products, fresh vegetables, and contaminated water
• Features Benefits
  Easy and Ready-to-use Kit., Detection: probe labelled with fluorescent dyes– Salmonella: HEX-BHQ1; Listeria: FAM-BHQ1a; IAC:CY5-BHQ2., Fast: results in less than 24 hours with minimal handling., Simultaneously detect and differentiate Salmonella spp. and Listeria monocytogenes in the same tube., Amplification limit: 10 UG per reaction (100%)., Inclusivity: Salmonella: Positive in 30 reference strains of food origin from the Spanish Type Culture Collection (CECT). Listeria: Positive in 48 reference strains of L. monocytogenes (serovars 1 to 7) from different collections., Reliable and highly specific Results., Exclusivity: Salmonella: Tested with 29 nontarget strains. Listeria: 100% Tested with 94 nontarget strains composed of 51 strains of Listeria nomonocytogenes and 43 strains of non-Listeria., Quantification Dynamic range: 6 logs., Quantification limit: 20 UG per reaction (100%).
• Storage Conditions
  -20°C
• Shipping Conditions
  Dry Ice
• Shelf life
  12 months
• Background
  The PCR-Salmonella-Listeria Detection kit is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragments from Salmonella spp and/or Listeria monocytogenes by the real-time PCR method (multiplex PCR). The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, mgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM (Listeria) and HEX fluorochrome (Salmonella), whereas the probe for the detection of IAC is labelled with the CY5 fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
• Data Sheet Link
• Additional description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
• Group
  PCR, polymerase chain reaction
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

• Gene target
  Salmonella-Listeria   Kit  
• Short name
  PCR-Salmonella-Listeria Kit
• Technique
  detection, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
• Species
  Listeria, Listeria, Salmonellas
• Alternative name
  PCR test kit-Salmonella-Listeria quantification reagent
• Alternative technique
  kits, dna-amplification
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Disease
  Listeria monocytogenes and Listeria species are gram-positive, rod-shaped, facultatively anaerobic, and non spore-forming. The major human pathogen in the Listeria genus is L. monocytogenes. It is usually the causative agent of the relatively rare bacterial disease listeriotic, listeriosis a serious infection caused by eating food contaminated with the bacteria. The disease affects pregnant women, newborns, adults with weakened immune systems, and the elderly. Antibodies and ELISA test kits for detection of listeria antigens. Salmonella typhimurium, enteriditis and Salmonella paratyphi antibodies or media detect this rod-shaped (bacillus) gram-negative bacteria of the Enterobacteriaceae family. The two species of Salmonella are Salmonella enterica and Salmonella bongori. Salmonella enterica is the type species and is further divided into six subspecies that include over 2500 serovars. S. enterica subspecies are found worldwide in all warm-blooded animals, and in the environment. S. bongori is restricted to cold-blooded animals particularly reptiles. Strains of Salmonella cause illnesses such as typhoid fever, paratyphoid fever, and food poisoning (salmonellosis).

MeSH Data

• Name
  Polymerase Chain Reaction
• Concept
  Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
• Tree numbers
  • E05.393.620.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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