PCR-Listeria monocytogenes Detection Kit

  • Catalog number
    K1449-96
  • Price
    639.13 USD
  • Size
    96 Rxns
  • Description
    PCR based Listeria monocytogenes Detection Kit
  • Summary
    PCR-Listeria monocytogenes Detection Kit is based on amplification and detection of specific DNA fragments from Listeria monocytogenes by real-time PCR method.
  • Detection Method
    Real-time PCR
  • Species Reactivity
    Listeria monocytogenes
  • Applications
    Real-time PCR
  • Sample Type
    Bacteria, Listeria monocytogenes, meat, poultry, dairy, vegetables, and ready to eat products., Food samples
  • Features Benefits
    Amplification limit: 10 UG per reaction (100%), 2 UG per reaction (40%), Inclusivity: Positive in 48 reference strains of L. monocytogenes (serovars 1 to 7) from different collections., Reliable and highly specific Results., Detection: probe labelled with fluorescent dyes Listeria monocytogenes: FAM-BHQ1a; IAC: HEXBHQ1., Fast: results in less than 24 hours with minimal handling., Quantification limit: 20 UG per reaction (100%), Quantification Dynamic range: 6 logs, Exclusivity: 100% Tested with 94 nontarget strains composed of 51 strains of Listeria nomonocytogenes and 43 strains of non-Listeria., Easy and Ready-to-use Kit.
  • Storage Conditions
    -20°C
  • Shipping Conditions
    Dry Ice
  • Shelf life
    12 months
  • Background
    BioVision’s PCR-Listeria monocytogenes Detection Kit is an ideal tool for simple, fast and reliable Amplification and Detection of specific DNA fragments from Listeria monocytogenes by the real-time PCR method. The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA polymerase, DNA-free water, mgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM, whereas the probe for the detection of IAC is labelled with the HEX fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR detection.
  • Additional description
    The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Gene target
  • Short name
    PCR-Listeria monocytogenes Kit
  • Technique
    detection, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
  • Species
    Listeria, Listeria
  • Alternative name
    PCR test kit-Listeria monocytogenes quantification reagent
  • Alternative technique
    kits, dna-amplification
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Disease
    Listeria monocytogenes and Listeria species are gram-positive, rod-shaped, facultatively anaerobic, and non spore-forming. The major human pathogen in the Listeria genus is L. monocytogenes. It is usually the causative agent of the relatively rare bacterial disease listeriotic, listeriosis a serious infection caused by eating food contaminated with the bacteria. The disease affects pregnant women, newborns, adults with weakened immune systems, and the elderly. Antibodies and ELISA test kits for detection of listeria antigens.
MeSH Data
  • Name
  • Concept
    Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
  • Tree numbers
    • E05.393.620.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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