Oxalate Decarboxylase, Active Bacterial Recombinant

• Catalog number
  7262-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  YvrK
• Alternative_names
  YvrK
• Description
  An enzyme which decomposes oxalic acid and oxalate
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Specific activity with 100 mM oxalate is approximately 150U/mg. (Specific activity is calculated by oxalate decarboxylase activity colorimetric assay kit (Cat. No. K664-100)
• Unit Definition
  : One unit is the amount of enzyme that generates 1.0 µmole of NADH at 37°C. Specific activity was expressed as U/mg protein.
• Molecular Weight
  45.9 kDa (384 aa + NT 6xHis-tag)
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  2 mg/ml
• Appearance
  Liquid
• Physical form description
  2 mg/mL solution in 20 mM Tris, pH 8 containing 20% glycerol.
• Background Information
  Oxalate decarboxylase (OxdC, EC4.1.1.2) is a manganese-containing enzyme, which decomposes oxalic acid and oxalate. With OxdC catalysis, oxalate is split into formate and CO2. This enzyme belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. The systematic name of this enzyme class is oxalate carboxy-lyase (formate-forming). This enzyme is also called oxalate carboxy-lyase. The enzyme is composed of two cupin domains, each of which contains a Mn (II) ion. This enzyme participates in glyoxylate and dicarboxylate metabolism. This enzyme has been recognized for diagnostics in diverse biotechnological applications such as the clinical assay of oxalate in blood and urine, therapeutics, process industry, and agriculture to lower oxalate levels in foods and the environment. The recombinant protein made from the Bacillus Subtilis sequence includes OxdC with N-terminal His-tag.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Oxalate   Decarboxylase   Active   Bacterial  
• Short name
  Oxalate Decarboxylase, Active Bacterial Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Oxalate Decarboxylase, functionnal Bacterial Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Radioallergosorbent Test
• Concept
  Scope note: An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
• Tree numbers
  • E01.370.225.812.735.830
  • E05.200.812.735.830
  • E05.478.566.380.810
  • E05.478.566.639.810
  • E05.478.594.760.830
  • E05.601.470.380.810
  • E05.601.470.639.810
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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