Ornithine Decarboxylase I (ODC1) Inhibitor Screening Kit (Colorimetric)
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Catalog number
K176-100
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Price
Please ask
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Size
100 assays
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Description
Convenient assay kit for rapid screening of potential ODC inhibitors
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Detection Method
Colorimetric (OD =340 nm)
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Species Reactivity
Human
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Applications
Screening for inhibitors of human Ornithine decarboxylase I (ODC1)
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Features Benefits
Fast and convenient,Simple procedure; takes ~ 30-60 minutes,The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples,Useful for screening possible inhibitors of human Ornithine decarboxylase I (ODC1)
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Ornithine decarboxylase (ODC) (EC 3.5.3.1) is a pyridoxal phosphate-dependent enzyme, which cleaves ornithine into putrescine and carbon dioxide. ODC is the initial step in the polyamine synthesis pathway. Putrescine, along with the subsequent polyamine products, spermidine and spermine has been shown to promote cell proliferation and arrest cell apoptosis in cancer. It is also known that proto-oncogenes, such as c-Myc can transcriptionally regulate this protein. Ornithine decarboxylase inhibitors, e.g. efluornithine (2-difluromethylornithine), alexidine and their analogs have been studied as ornithine and employed to irreversibly inhibit ODC and thus abolish cell growth by reducing intracellular polyamine that is required for the aforementioned cellular process. BioVision’s Ornithine Decarboxylase I (ODC1) Inhibitor Screening Kit is designed for screening potential human ODC1 inhibitors. DFMO is provided as an inhibitor control. The ODC1 activity is monitored by the decrease in absorbance readings (OD: 340 nm), while potential inhibitors will cause a decrease in the rate of absorbance change. The assay kit is simple, quick and can be used to identify and characterize ODC1 inhibitors in a high-throughput format.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Gene symbol
ODC1, SLC25A21, ODC1-DT
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Short name
Ornithine Decarboxylase I (ODC1) Inhibitor Screening Kit (Colorimetric)
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Technique
colorimetric, colorimetry
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Alternative name
Ornithine Decarboxylase I (ODC1) suppressor detection reagent (colorimetry)
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Alternative technique
assays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
Gene info
Product images
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