Non-Alcoholic Fatty Liver Disease (NAFLD) Detection Kit

• Catalog number
  K489-100
• Price
  683.69 USD
• Size
  100 assays

• Description
  Only kit on the market to measure Fatty liver Disease
• Summary
  • Detection method-Fluorescence (Ex/Em = 535/585 nm) & Absorbance (OD 570 nm) • Applications- Estimation of alanine:pyruvate ratio for the estimation of possible fatty liver disease - Estimation of NAFLD in biological samples - Determination of pyruvate and/ora alanine in biological samples
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm) & Absorbance (OD 570 nm)
• Applications
  • Estimation of pyruvate in biological samples • Estimation of alanine in biological samples • Determination of Non-Alcoholic Fatty liver disease in mammalian samples
• Sample Type
  • tissue: liver • biofluids: serum
• Features Benefits
  • Simple & Sensitive • High-Throughput adaptable • Suitable for detecting pyruvate and/or alanine (0.2-10 nmol) and Non-Alcoholic Fatty liver disease
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Non-Alcoholic Fatty Liver Disease (NAFLD; also known as Hepatosteatosis) is a disease caused by the accumulation of lipids in liver cells that is not related to alcohol usage. In order to reduce the use of invasive liver biopsies for the diagnosis of NAFLD progression, various biomarkers for hepatosteatosis has been evaluated. Recent studies on searching possible metabolic biomarkers for NAFLD, it was shown alanine to pyruvate ratio is significantly increased in rats that developed NAFLD. The result closely correlates with the increased activities of alanine aminotransferase, which is an established biomarker for liver damage. Thus, the measurement of alanine/pyruvate ratio in serum or liver could be a promising marker to detect NAFLD. BioVision’s NAFLD detection kit determines the alanine to pyruvate ratio in two independent enzymatic reactions. The difference between these two measurements gives the actual amount of alanine and pyruvate present in the samples. This kit provides a simple, fast and high throughput adaptable assay to measure the ratio of alanine and pyruvate with in various biological samples in both colorimetric (O.D. 570 nm) with detection range of alanine and pyruvate 2-10 nmol and fluorometric mode (Ex/Em = 535/587nm) with detection range 0.2-1 nmol.
• Data Sheet Link
• Additional description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

• Gene target
  Non-Alcoholic   Fatty   Liver   Disease   NAFLD   Kit  
• Short name
  Non-Alcoholic Fatty Liver Disease (NAFLD) Kit
• Technique
  detection
• Alternative name
  Non-Alcoholic Fatty Liver Disease (NAFLD) quantification reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  liver

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