Nickel Colorimetric Assay Kit
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Catalog numberK510-100
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Price:
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Size100 assays
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Summary• Detection method- Absorbance (300 to 600 nm) • Species reactivity- Mammalian • Applications- The assay is a simple method of quantifying Ni²ᶧ in a variety of samples, which gives a linear range of 2 to 50 nmol Nickel containing less than 25 nmol Cobalt.
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Sample TypeCell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
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Features Benefits• Simple procedure; takes less than ~40 minutes • Fast and convenient
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundNickel is one of four ferromagnetic elements (symbol Ni, at. Num. 28). Several enzymes depend on nickel for activity, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO₂ to CH₄) and some hydrogenases which allow the production or removal of H2. Most of these activities are found in the archaebacteria. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions. BioVision’s Nickel Assay kit provides a simple method of quantifying Ni²ᶧ in a variety of samples. The assay takes advantage of reaction of Ni²ᶧ with mercaptoethanol in borate buffer to form a complex with strong absorbance bands from ~300 to 600 nm. Fe²ᶧ and Co²ᶧ interfere with the assay, therefore extra steps (as described below) must be taken to subtract the interference in order to determine the correct Nickel concentration in mixed samples. Other ions tested (Mn²ᶧ, Cu²ᶧ, Zn²ᶧ) do not interfere with the assay, presumably no other ionic species are present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni²ᶧ in a variety of samples, which gives a linear range of 2 to 50 nmol Nickel containing less than 25 nmol Cobalt.
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TestBiovision supplies other types of Assays as 1.
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PropertiesColorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short nameNickel Colorimetric Assay Kit
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Techniquecolorimetric, Assay, assays, colorimetry
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HostAssay
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Alternative nameNickel colorimetry test reagent
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Alternative techniqueassays, arrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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