MTT Cell Proliferation Assay Kit (Colorimetric)

• Catalog number
  K299-1000
• Price
  Please ask
• Size
  1000 assays

• Description
  Non-radioactive and high-throughput method to measure cell proliferation/viability/cytotoxicity
• Summary
  • Detection- Absorbance (490 nm) • Applications: - Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients - Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals - Assessment of physiological mediators that inhibit cell growth
• Detection Method
  Absorbance (490 nm)
• Applications
  • Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients. • Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. • Assessment of physiological mediators that inhibit cell growth.
• Sample Type
  • Adherent and suspension cells • Proliferating and non-proliferating cells
• Features Benefits
  • Non-radioactive • High-throughput • The entire assay can be performed directly in a 96-well plate and does not require washing/harvesting/or solubilization steps.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Quantification of the number of viable cells is an indispensable tool in cell biology research. BioVision’s MTT Cell Proliferation Assay Kit is a sensitive method for quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product. Viable cells with active metabolism convert MTT into formazan, however, dead cells lose this ability. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance (590 nm) is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity, and apoptosis.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  MTT   Proliferation   Kit  
• Short name
  MTT Proliferation Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  MTT cellular expantion test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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