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Description
Using spin columns
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Summary
BioVision’s miRNA Extraction Kit is used to purify miRNA from cells and tissues. This kit is based on an improved guanidine thiocyanate- phenol one step method and elution of miRNA using spin columns. In this kit, the ribonucleases are inactivated and the genomic DNA, 18S and 28S RNAs are removed using spin columns. Cellular metabolite and proteins are removed by a series of elution-centrifugation steps. Subsequently, the microRNAs (including miRNA, snRNA, and other RNA less than 200 bp) are eluted from a second spin column in RNase-free water. The purified miRNA is ready for downstream applications including RNA sequencing, microarray, RT-PCR, Northern Blot etc.
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Detection Method
miRNA can be purified from cells and tissues using spin columns.
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Species Reactivity
N/A
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Applications
RNA sequencing, microarray, RT-PCR, Northern Blot
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Sample Type
Tissue and cell lysates
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Features Benefits
Purify miRNA, snRNA,and other RNA less than 200 bp,Genomic DNA is efficiently removed by buffer MRL,High quality Spin Columns with silica membranes,Highly pure miRNA for downstream applications
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Storage Conditions
Multiple Temperatures
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Shipping Conditions
RT
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Shelf life
12 months
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Background
BioVision’s miRNA Extraction Kit is used to purify miRNA from cells and tissues. This kit is based on an improved guanidine thiocyanate- phenol one step method and elution of miRNA using spin columns. In this kit, the ribonucleases are inactivated and the genomic DNA, 18S and 28S RNAs are removed using spin columns. Cellular metabolite and proteins are removed by a series of elution-centrifugation steps. Subsequently, the microRNAs (including miRNA, snRNA, and other RNA less than 200 bp) are eluted from a second spin column in RNase-free water. The purified miRNA is ready for downstream applications including RNA sequencing, microarray, RT-PCR, Northern Blot etc.
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