Minimal Inhibitory Concentration Kit

• Catalog number
  K195-2500
• Price
  Please ask
• Size
  2500 assays

• Description
  For Gram Negative Rods
• Summary
  BioVision’s Minimal Inhibitory Concentration (MIC) kit, testing for Gram Negative Rods (GNR) is a broth microdilution assay that is simple yet sensitive for the quantification of any antimicrobial agent against a given bacterial isolate. The antibiotic efficacy is quantified using a Water Soluble Tetrazolium salt (WST) which changes color reflecting the viability of the bacteria as a response to antibiotic treatment. The test is performed in a microplate with bacteria-inoculated-Luria Bertani (LB) broth and serial dilutions of antimicrobial agents can be added. Our kit includes four antibiotics often prescribed for infections caused by gram negative rods. They are: Ampicillin Sodium, Chloramphenicol, Enrofloxacin, and Gentamicin Sulfate. Vancomycin Hydrochloride, an antibiotic effective in the treatment of gram positive cocci, is included as a negative control. The Antibiotic Susceptibility Limits of these antibiotics have been evaluated and correlated with published standards (Andrews, 2001) for Minimum Inhibitory Concentrations.
• Detection Method
  Absorbance, OD 460 nm
• Species Reactivity
  Gram Negative Rods, E. coli or other gram negative bacteria
• Sample Type
  E. coli grown in LB. Pseudomonas aeruginosa, Staphylococcus aureus have not been evaluated, but are commonly tested.
• Features Benefits
  Broth Microdilution Assay, Quantification of any antimicrobial agent against a given bacterial isolate, Sensitive, Simple
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Testing antibiotic susceptibility in bacterial isolates enables the detection of drug resistance in common bacterial pathogens. This ensures susceptibility to selected pharmaceutical interventions for specific pathogens classified as gram negative rods. The broth microdilution, a well-known method in microbiology is flexible and provides quantitative and semi-quantitative results to monitor resistance amongst bacterial pathogens. It is called microdilution because it involves the use of small volumes of broth dispensed in sterile, plastic microdilution trays (~0.1 ml of broth). BioVision’s Minimal Inhibitory Concentration (MIC) kit, testing for Gram Negative Rods (GNR) is a broth microdilution assay that is simple yet sensitive for the quantification of any antimicrobial agent against a given bacterial isolate. The antibiotic efficacy is quantified using a Water Soluble Tetrazolium salt (WST) which changes color reflecting the viability of the bacteria as a response to antibiotic treatment. The test is performed in a microplate with bacteria-inoculated-Luria Bertani (LB) broth and serial dilutions of antimicrobial agents can be added. Our kit includes four antibiotics often prescribed for infections caused by gram negative rods. They are: Ampicillin Sodium, Chloramphenicol, Enrofloxacin, and Gentamicin Sulfate. Vancomycin Hydrochloride, an antibiotic effective in the treatment of gram positive cocci, is included as a negative control. The Antibiotic Susceptibility Limits of these antibiotics have been evaluated and correlated with published standards (Andrews, 2001) for Minimum Inhibitory Concentrations.
• Data Sheet Link

• Gene target
  Minimal   Inhibitory   Concentration   Kit  
• Short name
  Minimal Inhibitory Concentration Kit
• Alternative name
  Minimal Inhibitory Concentration reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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