Lipid Peroxidation Colorimetric Assay Kit

• Catalog number
  K454-100
• Price
  Please ask
• Size
  100 assays

• Description
  Lipid Peroxidation Colorimetric Assay Kit
• Summary
  BioVision’s Lipid Peroxidation Assay Kit uses a proprietary set of reagents that minimizes the interference from other lipid peroxidation products including 4-hydroxyalkenals. The principle is based on the reaction between a specific chromogenic reagent and MDA at 45 °C. The assay is simple to perform, and yields a strong, yet stable colorimetric signal (OD 586 nm) that is proportional to the amount of MDA in samples. Our assay can detect as low as 3 nmol MDA in samples.
• Detection Method
  Colorimetric assay (OD 586 nm)
• Species Reactivity
  All
• Sample Type
  Plasma, Serum, Tissue and cell lysates
• Features Benefits
  Specific, Simple procedure, Can detect as low as 3 nmol MDA in samples, Fast and convenient
• Storage Conditions
  4°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Lipid peroxidation is a type of oxidative damage that affects cellular membranes, lipoproteins, and other lipid-containing molecules. This series of chain reactions occur between highly reactive oxygen species and unsaturated fatty acids in cell membranes, which leads to cell damage. Lipid peroxides, derived from polyunsaturated fatty acids are unstable and decompose forming malondialdehyde (MDA) and 4-hydroxyalkenals. The measurement of MDA is widely used and is considered as an indicator of lipid peroxidation. Traditionally, 2-Thiobarbituric acid (TBA) method has been widely used for the colorimetric detection and measurement of MDA in samples. However, this reaction is relatively nonspecific since both free and protein-bound MDA react with TBA. BioVision’s Lipid Peroxidation Assay Kit uses a proprietary set of reagents that minimizes the interference from other lipid peroxidation products including 4-hydroxyalkenals. The principle is based on the reaction between a specific chromogenic reagent and MDA at 45 °C. The assay is simple to perform, and yields a strong, yet stable colorimetric signal (OD 586 nm) that is proportional to the amount of MDA in samples. Our assay can detect as low as 3 nmol MDA in samples. MDA + Chromogenic Reagent= Chromophore (OD 586 nm)
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Lipid   Peroxidation   Kit  
• Short name
  Lipid Peroxidation Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Lipid Peroxidation colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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