LDL Uptake Assay Kit (Cell-Based)
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Catalog number
K436-100
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Price
Please ask
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Size
100 assays
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Description
Sensitive assay for the measurement of LDL
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Summary
Easy-to-follow protocol for the measurement of LDL uptake in mammalian cell lines.
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Detection Method
Fluorometric (Ex/Em= 488/523 nm)
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Species Reactivity
All
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Applications
Screening and characterization of drugs or treatment conditions that inhibit or stimulate LDL uptake,Characterization of LDL uptake in different cell types.
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Sample Type
Adherent Cell Line (e.g. HepG2 or Huh-7 cells)
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Features Benefits
Measure LDL uptake in different cell types,Non-Radioactive,Contains fluorescent human LDL, which is covalently-labeled with a bright, highly photostable fluorophore,Screen, study or characterize stimulators or inhibitors of LDL uptake
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 moths
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Background
Low-density lipoprotein (LDL) is a large spherical macromolecule that serves as a ‘shuttle’ to transport hydrophobic molecules (lipids and cholesterol) throughout the body via the aqueous environment of the bloodstream. An LDL particle consists of a hydrophobic core of cholesterol esters (with long-chain fatty acids linked to the hydroxyl group) and triglycerides surrounded by an amphipathic shell containing the 510 kDa protein Apolipoprotein B-100, various phospholipids and non-esterified cholesterol molecules. LDL is taken up by cells such as hepatocytes, macrophages, and endothelial cells by specific receptor-mediated endocytosis. High levels of LDL have been associated with arterial inflammation, which leads to cardiovascular diseases (CVD) like atherosclerosis, ischemic stroke and myocardial infarction. LDL particles can migrate through inflamed vascular endothelial tight junctions, where they become oxidized and attract circulating monocytes. Monocytes differentiate into activated macrophages, which further accumulate LDL and become foam cells, leading to arterial wall thickening/scaring and formation of fibrous fatty plaques. Current CVD preventative therapies include decreasing LDL with statins, which prevent endogenous cholesterol biosynthesis by inhibiting H mg-CoA reductase. However, drugs that inhibit LDL uptake by macrophages or enhance LDL clearance by increasing hepatic LDL reuptake are also being investigated. BioVision’s LDL Uptake Assay Kit enables measurement of receptor-mediated LDL uptake by various cell types. The kit contains fluorescent human LDL, which is covalently-labeled with a bright, highly photostable fluorophore that can be detected when taken up by cells (Ex/Em = 488/523 nm). Unlabeled human LDL is included in the kit for validation of specific labeled LDL uptake. The kit contains a complete set of reagents sufficient for performing 100 reactions and a 96-well polylysine-coated black cell culture plate with a thin, microscopy-grade clear bottom.
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Test
Biovision supplies other types of Assays as 1.
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbol
PLA2G7
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Short name
LDL Uptake Assay Kit ( -Based)
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Technique
Assay, assays
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Host
Assay
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Alternative name
LDL Uptake test reagent (cellular-Based)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell
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Gene info
Product images
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