IL17 A/F, Rat Recombinant

• Catalog number
  7587-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Interleukin 17 A/F, IL-17A, IL-17F, Interleukin 17A, Interleukin 17F
• Alternative_names
  Interleukin 17 A/F, IL-17A, IL-17F, Interleukin 17A, Interleukin 17F
• Description
  Induces the production of a variety of chemokines
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Endotoxin Level
  Measured by kinetic LAL analysis and is typically ≤ 1 EU/ug protein
• Activity Specifications test method
  The activity determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts, is typically 20-30 ng/mL.
• Biological activity
  The activity determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts, is typically 20-30 ng/mL.
• Molecular Weight
  30.7 kDa.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized without any additives.
• Reconstitution Instructions
  Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
• Background Information
  Interleukin-17AF (IL-17AF) is a member of the IL-17 family of proteins produced by a subset of T cells, called Th17, following stimulation with IL-23. Since IL-17AF is thought to signal through the IL-17R receptor, its biological function is similar to that of IL-17A in that it induces the production of a variety of chemokines, in addition to airway neutrophilia. In regard to these functions, IL-17AF has less activity than the IL-17A homodimer but, greater activity than the IL-17F homodimer. Human and rat IL-17AF both show activity on mouse cells. Recombinant rat IL-17AF is a non-glycosylated, disulfide-linked heterodimer. It is comprised of one monomeric subunit each of IL-17A and IL-17F, having a total of 269 amino acids and a molecular weight of 30.7 kDa.
• Amino acid sequence
  IL-17A subunit: MAVLIPQSSV CPNAEANNFL QNVKVNLKVL NSLSSKASSR RPSDYLNRST SPWTLSRNED PDRYPSVIWE AQCRHQRCVN AEGKLDHHMN SVLIQQEILV LKREPEKCPF TFRVEKMLVG VGCTCVSSIV RHAS IL-17F subunit: MARRNPKVGL SALQKAGNCP PLEDNSVRVD IRIFNQNQGI SVPRDFQNRS SSPWD YNITR DPDRFPSEIA EAQCRHSGCI NAQGQEDGSM NSVPIQQEIL VLRREPQGCS NSFRLEKMLI KVGCTCVTPI VHHAA
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  IL17   A/F  
• Gene symbol
  IL17RC, IL17A
• Short name
  IL17 A/F, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  IL17 A/F, Rat Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:18358
• Gene
  IL17RC
• Long gene name
  interleukin 17 receptor C
• Synonyms
  • IL17-RL
• GenBank acession
  • BC006411
• Locus
  3p25.3
• Discovery year
  2003-07-07
• Entrez gene record
  84818
• Pubmed identfication
  • 11706037
• RefSeq identity
  • NM_032732
• Classification
  • Interleukin receptors
• VEGA ID
  OTTHUMG00000128648
• Locus Specific Databases
  • LRG_1311

Gene info

• Identity
  HGNC:5981
• Gene
  IL17A
• Long gene name
  interleukin 17A
• Synonyms gene
  • CTLA8
  • IL17
• Synonyms gene name
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• Synonyms
  • IL-17A
  • IL-17
• Synonyms name
  • cytotoxic T-lymphocyte-associated protein 8
• GenBank acession
  • U32659
• Locus
  6p12.2
• Discovery year
  1993-10-25
• Entrez gene record
  3605
• Pubmed identfication
  • 8390535
• RefSeq identity
  • NM_002190
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000014840
• Locus Specific Databases
  • LRG_1223

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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