Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit

  • Catalog number
    K265-200
  • Price
    Please ask
  • Size
    200 assays
  • Description
    Sensitive Assay, HTS
  • Summary
    • Detection method- Absorbance (570 nm)and fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Applications- The kit provides a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring H₂O₂ in biological samples.
  • Sample Type
    Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
  • Features Benefits
    • Simple procedure; takes less than ~1 hours • Fast and convenient • The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H₂O₂ in the sensitive fluorometric assay.
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Hydrogen Peroxide is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-κB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases. BioVision’s Hydrogen Peroxide Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H₂O₂ in biological samples. In the presence of Horse Radish Peroxidase (HRP), the OxiRed Probe reacts with H₂O₂ to produce product with color (λmax = 570 nm) and red-fluorescent (Ex/Em=535/587 nm). The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H₂O₂ in the sensitive fluorometric assay.
  • Test
    Biovision supplies other types of Assays as 1.
  • Properties
    Colorimetric assays or detection use UV absorption or enzymatic color reaction.
  • Gene target
  • Short name
    Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit
  • Technique
    colorimetric, Assay, assays, colorimetry
  • Host
    Assay
  • Alternative name
    Hydrogen Peroxide colorimetry/fluorescence test reagent
  • Alternative technique
    assays, arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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