Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit
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Catalog number
K265-200
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Price
Please ask
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Size
200 assays
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Description
Sensitive Assay, HTS
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Summary
• Detection method- Absorbance (570 nm)and fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Applications- The kit provides a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring H₂O₂ in biological samples.
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Sample Type
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
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Features Benefits
• Simple procedure; takes less than ~1 hours • Fast and convenient • The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H₂O₂ in the sensitive fluorometric assay.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Hydrogen Peroxide is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-κB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases. BioVision’s Hydrogen Peroxide Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H₂O₂ in biological samples. In the presence of Horse Radish Peroxidase (HRP), the OxiRed Probe reacts with H₂O₂ to produce product with color (λmax = 570 nm) and red-fluorescent (Ex/Em=535/587 nm). The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H₂O₂ in the sensitive fluorometric assay.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Hydrogen Peroxide colorimetry/fluorescence test reagent
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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