Human recombinant Ubiquitin Activating Enzyme E1

• Catalog number
  6429-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Human recombinant Ubiquitin Activating Enzyme E1
• Alternative_names
  UBE1, UBA1, POC20, A1S9T, A1ST, AMCX1, GXP1, SMAX2
• Description
  First enzyme involved in ubiquitin conjugation pathway, conjugating ubiquitin to the substrate.
• Recombinant
  Yes
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Typical enzyme concentration of 5 nM – 100 nM is used for in vitro conjugation, depending on conditions.
• Molecular Weight
  118 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.
• Background Information
  UBE1, the ubiquitin activating enzyme, is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Ubiquitin   Activating  
• Gene symbol
  UBA6, UBA7, UBA5
• Short name
  recombinant Ubiquitin Activating Enzyme E1
• Technique
  Recombinant, Enzyme, enzymes, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. Ubiquitin Activating Enzyme E1
• Alternative technique
  rec, enzymes

Gene info

• Identity
  HGNC:25581
• Gene
  UBA6
• Long gene name
  ubiquitin like modifier activating enzyme 6
• Synonyms gene
  • UBE1L2
• Synonyms gene name
  • ubiquitin-activating enzyme E1-like 2
  • ubiquitin-like modifier activating enzyme 6
• Synonyms
  • FLJ10808
• Synonyms name
  • UBA6, ubiquitin-activating enzyme E1
• GenBank acession
  • AK094164
• Locus
  4q13.2
• Discovery year
  2006-02-14
• Entrez gene record
  55236
• Pubmed identfication
  • 17580310
• RefSeq identity
  • NM_018227
• Classification
  • Ubiquitin like modifier activating enzymes
• VEGA ID
  OTTHUMG00000129299

Gene info

• Identity
  HGNC:12471
• Gene
  UBA7
• Long gene name
  ubiquitin like modifier activating enzyme 7
• Synonyms gene
  • UBE1L
• Synonyms gene name
  • ubiquitin-activating enzyme E1-like
  • ubiquitin-like modifier activating enzyme 7
• Synonyms
  • D8
  • UBE2
  • UBA1B
• Synonyms name
  • UBA1, ubiquitin-activating enzyme E1 homolog B (yeast)
  • UBA7, ubiquitin-activating enzyme E1
• GenBank acession
  • BC006378
• Locus
  3p21.31
• Discovery year
  1993-11-24
• Entrez gene record
  7318
• Pubmed identfication
  • 8327486
• RefSeq identity
  • NM_003335
• Classification
  • Ubiquitin like modifier activating enzymes
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000158267

Gene info

• Identity
  HGNC:23230
• Gene
  UBA5
• Long gene name
  ubiquitin like modifier activating enzyme 5
• Synonyms gene
  • UBE1DC1
• Synonyms gene name
  • ubiquitin-activating enzyme E1-domain containing 1
  • ubiquitin-like modifier activating enzyme 5
• Synonyms
  • FLJ23251
• Synonyms name
  • UBA5, ubiquitin-activating enzyme E1 homolog (yeast)
• GenBank acession
  • AY253672
• Locus
  3q22.1
• Discovery year
  2003-10-28
• Entrez gene record
  79876
• Pubmed identfication
  • 11230166
  • 15071506
• RefSeq identity
  • NM_024818
• Classification
  • Ubiquitin like modifier activating enzymes
• VEGA ID
  OTTHUMG00000159759

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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