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Synonyms
TNFRSF10B, TRAILR2, TRAIL-R2, CD262,DR5, KILLER, TRICK2, ZTNFR9, TRICKB
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Alternative_names
TNFRSF10B, TRAILR2, TRAIL-R2, CD262,DR5, KILLER, TRICK2, ZTNFR9, TRICKB
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Description
Promotes the activation of NF-kappa-B
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Recombinant
True
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Source
HEK 293 Cells
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Purity by SDS PAGE
>90%
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Assay
SDS-PAGE
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Endotoxin Level
Less than 1.0 EU per ug by the LAL method.
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Molecular Weight
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.1 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Storage Temp
-20ºC
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized powder
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Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
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Background Information
Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TNFRSF10B / DR-5 is widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines. TRAILR2 / CD262 / DR5 is the receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD (a death domain containing adaptor protein) of TRAIL-R2 / TNFRSF10B recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. CD262 / DR5 Promotes the activation of NF-kappa-B. DR5 is essential for ER stress-induced apoptosis and is regulated by p53/TP53.
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Amino acid sequence
AA Asn 53 - Lys 180
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Handling
Centrifuge the vial prior to opening.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TNFRSF10B, TNFSF10
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Short name
CellExp™ TRAIL R2 / TNFRSF10B, Mouse recombinant
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Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
mouse
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Species
Humans, Mouses
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Alternative name
H. sapiens CellExp™ TRAIL R2 / tumor necrosis factor receptor superfamily, member 10b, Mouse Rec.
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Alternative technique
rec, murine
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Alternative to gene target
tumor necrosis factor receptor superfamily, member 10b, CD262 and DR5 and KILLER and KILLER/DR5 and TRAIL-R2 and TRAILR2 and TRICK2 and TRICK2A and TRICK2B and TRICKB and ZTNFR9, TNFRSF10B and IDBG-11376 and ENSG00000120889 and 8795, TRAIL binding, Plasma membranes
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