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Synonyms
Urokinase, PLAU, ATF, UPA, URK, u-PA, BDPLT5, QPD
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Alternative_names
Urokinase, PLAU, ATF, UPA, URK, u-PA, BDPLT5, QPD
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Description
A potent marker of invasion and metastasis in a variety of human cancers
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Recombinant
1
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Source
HEK 293 cells
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Purity by SDS PAGE
>90%
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Endotoxin Level
Less than 1.0 EU per ug by the LAL method.
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Activity Specifications test method
Measured by it's ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2,000 pmol/min/µg, as measured under the described conditions.
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Molecular Weight
This protein carries a polyhistidine tag at the C-terminus. The active form of Human PLAU is a disulfide-linked heterodimer composed of long chain A (Ser 21 - Phe 177) and chain B (Ile 179 - Leu 431) with calculated MW of 17.8 kDa and 29.2 kDa. The long chain A is further cleaved to yield a short chain A (Lys 156 - Phe 177) and N-terminal fragment (Ser 21 - Lys 155) with calculated MW of 15.3 kDa. The protein migrates as 17 kDa (N-terminal fragment), 32-35 kDa (chain B) and 45-50 kDa (long chain A & chain B) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
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Storage Temp
-20ºC
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in deionized water. Do not vortex. For extended storage, it is recommended to store at -70°C
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Background Information
Urokinase-type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).
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Amino acid sequence
aa Ser 21 - Leu 431
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
PLAU
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Short name
CellExp™ PLAU / uPA (Activated), Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ plasminogen activator, urokinase / uPA (Activated), H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
plasminogen activator, urokinase, ATF and BDPLT5 and QPD and u-PA and UPA and URK, PLAU and IDBG-78889 and ENSG00000122861 and 5328, protein binding, Extracellular, Plau and IDBG-136606 and ENSMUSG00000021822 and 18792, PLAU and IDBG-630603 and ENSBTAG00000005947 and 281408
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