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Synonyms
Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, GPC3, OCI6
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Alternative_names
GPC3, OCI5, Glypican-3, GTR2-2, MXR7, DGSX, SDYS, SGB, SGBS, SGBS2
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Description
Cell surface proteoglycan that inhibits the dipeptidyl peptidase activity of DPP4 and involved in modulation of IGF2 interactions with its receptor.
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
> 90%
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Assay
SDS-PAGE
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Measured by its binding ability with FGF-2 in a functional ELISA. Human Glypican 3 (Cat. No. P1057) can bind Immobilized FGF-2 (Cat. No. 4037 at 0.05 ug/well) with a linear range of 2.5-600 ng/mL.
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Binding Capacity
Measured by its binding ability with FGF-2 in a functional ELISA. Human Glypican 3 (Cat. No. P1057) can bind Immobilized FGF-2 (Cat. No. 4037 at 0.05 ug/well) with a linear range of 2.5-600 ng/mL.
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Molecular Weight
61.7 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4 with 5% Trehalose added as protectant before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile PBS, pH 7.
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Background Information
Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
GPC3-AS1, GPC3
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Short name
CellExp™ Glypican 3 / GPC3, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ Glypican 3 / glypican 3, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
glypican 3, DGSX and GTR2-2 and MXR7 and OCI-5 and SDYS and SGB and SGBS and SGBS1, GPC3 and IDBG-86754 and ENSG00000147257 and 2719, peptidyl-dipeptidase inhibitor activity, Extracellular, Gpc3 and IDBG-145213 and ENSMUSG00000055653 and 14734, GPC3 and IDBG-630953 and ENSBTAG00000020406 and 615239
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