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Synonyms
TNFSF18, AITRL, TL6, hGITRL, GITR Ligand
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Alternative_names
TNFSF18, AITRL, TL6, hGITRL, GITR Ligand
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Description
Tumor necrosis factor receptor superfamily member 18
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
> 95%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per/ug
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Immobilized Mouse GITR, His Tag at 10ug/mL (100 uL/well) can bind Mouse GITR Ligand, Fc Tag with a linear range of 0.007-0.256 ug/mL.
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Binding Capacity
Immobilized Mouse GITR, His Tag at 10ug/mL (100 uL/well) can bind Mouse GITR Ligand, Fc Tag with a linear range of 0.007-0.256 ug/mL.
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Molecular Weight
41.4 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
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Background Information
Tumor necrosis factor ligand superfamily member 18 (TNFSF18) is also known as Glucocorticoid - induced TNF-related ligand (GITRL or GITR Ligand), Activation - inducible TNF - related ligand (AITRL), which belongs to the tumor necrosis factor family. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. TNFSF18 / GITRL is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells. TNFSF18 also mediates activation of NF-kappa-B.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TNFRSF18, TNFSF18
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Short name
CellExp™ GITR Ligand / TNFSF18 Fc Tag, Mouse recombinant
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Technique
Recombinant, Mouse, FC, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
mouse
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Label
Fc Tag
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Species
Humans, Mouses
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Alternative name
H. sapiens CellExp™ GITR Ligand / tumor necrosis factor (ligand) superfamily, member 18 fragment c detection labelled, Mouse Rec.
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Alternative technique
rec, murine
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Alternative to gene target
tumor necrosis factor (ligand) superfamily, member 18, AITRL and GITRL and hGITRL and TL6, TNFSF18 and IDBG-104861 and ENSG00000120337 and 8995, tumor necrosis factor receptor superfamily binding, Extracellular, Tnfsf18 and IDBG-201543 and ENSMUSG00000066755 and 240873, LOC768081 and IDBG-632315 and ENSBTAG00000047412 and 768081
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