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Synonyms
CD3E & CD3D, CD3 delta & CD3 epsilon, CD3 delta / epsilon
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Alternative_names
CD3E & CD3D, CD3 delta & CD3 epsilon, CD3 delta / epsilon
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Description
T cell receptor plays an important role in coupling antigen recognition
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Recombinant
Yes
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Source
Expi293 cells
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Purity by SDS PAGE
>95%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per/ug
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Human CD3 epsilon & CD3 delta Heterodimer Protein was immobilized at 0.5 µg/ml (100 µl/well). Dose response curve using Anti-hCD3E/CD3D Ab foralumab gave an EC₅₀ of 21.69 ng/ml by ELISA. Human CD3 epsilon & CD3 delta Heterodimer Protein was Immobilized at 0.1 µg/ml (100 µl/Well). Dose response curve using Anti-hCD3E/CD3D Ab OKT3 gave an EC₅₀ of 21 ng/ml by ELISA.
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Binding Capacity
Immobilized OKT3 MAb at 5 ug/mL (100 uL/well) can bind Human CD3E & CD3D, His Tag with a linear range of 0.625-5 ug/mL
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Molecular Weight
16.7 kDa and 13.5 kDa
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Storage Temp
-80°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS pH 7.4. Generally 5-8% Mannitol or Trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
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Background Information
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
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Amino acid sequence
CD3E (Asp 23-Asp 126) and CD3D (Phe 22-Ala 105) (Fc tag at C-terminal)
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
