Histone H4 Antibody
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Catalog number3624-100
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PricePlease ask
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Size100 µg
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Antibody TargetHistone H4
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Target Alternative NameH4FB , HIST1H4B , HIST1H4F, H4FN, H4FH
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide corresponding to the N-terminus of human Histone H4
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AccessionCAB02549
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Gene Id8367
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) affinity purified rabbit anti-Histone H4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation, Immunohistochemistry
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Application And UsagesWestern blotting (0.5-4 µg/ml), in immunoprecipitation (20-40 µg/ml) and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~11 kDa histone H4 protein. It does not cross-react with other histones.
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DescriptionThe nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolH4C8, H4-16, H4C14
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Short nameHistone H4 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameHistone H4 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameH4 clustered histone 8
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Synonyms gene
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Synonyms gene name
- H4 histone family, member H
- histone 1, H4h
- histone cluster 1, H4h
- histone cluster 1 H4 family member h
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H4 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH4 histone 16
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Synonyms gene
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Synonyms gene name
- histone 4, H4
- histone cluster 4, H4
- histone cluster 4 H4
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H4 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH4 clustered histone 14
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Synonyms gene
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Synonyms gene name
- H4 histone, family 2
- histone 2, H4
- histone 2, H4a
- histone cluster 2, H4a
- histone cluster 2 H4 family member a
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GenBank acession
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Locus
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Discovery year1998-09-07
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Entrez gene record
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Pubmed identfication
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Classification
- H4 histones
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data