His-Tag Antibody (Clone 11E12)

• Catalog number
  6714-100
• Price
  Please ask
• Size
  100 μl

• Antibody Target
  His-Tag
• Target Alternative Name
  His-Tag
• Antibody Type
  Monoclonal
• Clone
  Clone 11E12
• Species Reactivities
  All
• Immunogen Sequence
  6X-HIS-KLH
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1 mg/ml
• Formulation
  1 mg/ml in 0.15 M PBS
• Purification
  Protein A chromatography from in vitro production
• Application
  WB, ELISA
• Positive Control
  Western blot: His-Tagged protein
• Application And Usages
  ELISA, WB
• Description
  A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  histidine
• French translation
  anticorps

• Gene target
  His-Tag   11E12  
• Short name
  His-Tag Antibody (Clone 11E12)
• Technique
  Antibody, antibodies against human proteins, antibodies for, Tags
• Isotype
  Mouse IgG1 κ
• Label
  His
• Alternative name
  histidine-detection labelled (antibody to-) (clonality 11E12)
• Alternative technique
  antibodies
• Clone name
  11E12

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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