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Description
Using spin columns
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Summary
BioVision’s Gel and PCR DNA Purification Kit allows the purification of DNA from agarose gels, PCR, RFLP, phosphorylation, labeling and other enzymatic reactions. In this kit, DNA fragments bind to the mini columns and the DNA fragments are eluted in an elution buffer. The purified DNA is ready for downstream applications including Sequencing and Restriction Enzyme Digestion.
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Detection Method
DNA can be recovered or concentrated from Agarose gels, PCR, RFLP, phosphorylation, labeling and other enzymatic reactions using spin columns.
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Species Reactivity
N/A
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Applications
Sequencing, Restriction Enzyme Digestion
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Sample Type
Agarose gels, Restriction Enzyme Digestion products, PCR, RFLP, phosphorylation, labeling and other enzymatic reactions
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Features Benefits
High quality spin columns, All the centrifugation steps can be performed at room temperature, High Efficiency: > 90% recovery of DNA, Broad Fragment Size Range: 200 bp-20 kb, Fast and Reliable
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Storage Conditions
RT
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Shipping Conditions
RT
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Shelf life
12 months
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Background
BioVision’s Gel and PCR DNA Purification Kit allows the purification of DNA from agarose gels, PCR, RFLP, phosphorylation, labeling and other enzymatic reactions. In this kit, DNA fragments bind to the mini columns and the DNA fragments are eluted in an elution buffer. The purified DNA is ready for downstream applications including Sequencing and Restriction Enzyme Digestion.
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Test
A gel is a solid jelly-like material that can have properties ranging from soft and weak to hard and tough. Gels are defined as a substantially dilute cross-linked system, which exhibits no flow when in the steady-state. By weight, gels are mostly liquid, yet they behave like solids due to a three-dimensional cross-linked network within the liquid. It is the crosslinking within the fluid that gives a gel its structure (hardness) and contributes to the adhesive stick (tack). In this way gels are a dispersion of molecules of a liquid within a solid in which the solid is the continuous phase and the liquid is the discontinuous phase. The word gel was coined by 19th-century Scottish chemist Thomas Graham by clipping from gelatin. Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
PCR DNA Purification kit
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Technique
Purification, dna, Gel, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. purified
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Alternative name
electrophoretic matrix and PCR test kit Desoxyribonucleic acid purification reagent
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Alternative technique
purifications, kits, dna-amplification
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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