GalNAc-T2, soluble fragment, Human Recombinant

• Catalog number
  P1215-5
• Price
  Please ask
• Size
  5 ug

• Synonyms
  Polypeptide N-acetylgalactosaminyltransferase 2, Polypeptide GalNAc transferase 2, GaNTase 2
• Alternative_names
  Polypeptide N-acetylgalactosaminyltransferase 2, Polypeptide GalNAc transferase 2, GaNTase 2
• Description
  Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis
• Recombinant
  Yes
• Source
  Insect cells
• Purity by SDS PAGE
  > 90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by transfer of GalNAc from UDP-GalNAc to the peptide MEELGMAPALQPTQGAMPAF.
• Biological activity
  Specific activity >300 pmol/min/ug
• Results
  >300 pmol/min/ug
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Concentration
  360 ug/ml
• Appearance
  Liquid
• Physical form description
  Sterile filtered solution in 20 mM Hepes pH 7 and 100 mM NaCl
• Background Information
  Polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2) catalyzes the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the hydroxyl group of serine and threonine residues. Twenty GalNAc-T genes have been identified in humans and most have been shown to represent active GalNAc-Ts. All isoforms are type II transmembrane proteins, with different but partly overlapping substrate preferences. The GalNAc-Ts control the initiation of mucin-type O-linked glycosylation and determine the location and density of O-glycans in a protein. Addition of GalNAc to an unglycosylated Ser/Thr residue creates the Tn antigen GalNAca1-S/T, and subsequent addition of sialic acid by ST6GalNAc-I forms the cancer associated STn antigen. GalNAc-T2 is primarily located in Golgi, and is widely distributed in human tissues.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GalNAc-T2   soluble  
• Gene symbol
  GALNT2, HBA2, B3GALT2, HBG1, B4GALT2, ACTRT2, SLC25A5, HBZP1, B3GNT2
• Short name
  GalNAc-T2, soluble , Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Isotype
  Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
• Species
  Human, Humans
• Alternative name
  GalNAc-T2, soluble fragment, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4124
• Gene
  GALNT2
• Long gene name
  polypeptide N-acetylgalactosaminyltransferase 2
• Synonyms gene name
  • UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
• Synonyms
  • GalNAc-T2
• Synonyms name
  • polypeptide GalNAc transferase 2
• GenBank acession
  • BC041120
• Locus
  1q42.13
• Discovery year
  1996-10-26
• Entrez gene record
  2590
• Pubmed identfication
  • 9592121
  • 7592619
• RefSeq identity
  • NM_004481
• Classification
  • Polypeptide N-acetylgalactosaminyltransferases
• VEGA ID
  OTTHUMG00000037771

Gene info

• Identity
  HGNC:4824
• Gene
  HBA2
• Long gene name
  hemoglobin subunit alpha 2
• Synonyms gene name
  • hemoglobin, alpha 2
• Synonyms
  • HBA-T2
• GenBank acession
  • BC008572
• Locus
  16p13.3
• Discovery year
  2001-06-22
• Entrez gene record
  3040
• Pubmed identfication
  • 6452630
  • 2649166
• RefSeq identity
  • NM_000517
• Classification
  • Hemoglobin subunits
• VEGA ID
  OTTHUMG00000059924
• Locus Specific Databases
  • HbVar: A Database of Human Hemoglobin Variants and Thalassemias
  • The Globin Gene Server
  • LRG_1240

Gene info

• Identity
  HGNC:917
• Gene
  B3GALT2
• Long gene name
  beta-1,3-galactosyltransferase 2
• Synonyms gene name
  • UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2
  • UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase 2
• Synonyms
  • beta3Gal-T2
• GenBank acession
  • Y15060
• Locus
  1q31.2
• Discovery year
  1998-12-01
• Entrez gene record
  8707
• Pubmed identfication
  • 9582303
  • 9417100
• RefSeq identity
  • NM_003783
• Classification
  • Beta 3-glycosyltransferases
• VEGA ID
  OTTHUMG00000035687

Gene info

• Identity
  HGNC:4831
• Gene
  HBG1
• Long gene name
  hemoglobin subunit gamma 1
• Synonyms gene name
  • hemoglobin, gamma A
• Synonyms
  • HBG-T2
• GenBank acession
  • M91036
• Locus
  11p15.4
• Discovery year
  2001-06-22
• Entrez gene record
  3047
• Pubmed identfication
  • 2649166
• RefSeq identity
  • NM_000559
• Classification
  • Hemoglobin subunits
• VEGA ID
  OTTHUMG00000066681
• Locus Specific Databases
  • HbVar: A Database of Human Hemoglobin Variants and Thalassemias
  • The Globin Gene Server

Gene info

• Identity
  HGNC:925
• Gene
  B4GALT2
• Long gene name
  beta-1,4-galactosyltransferase 2
• Synonyms gene name
  • UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2
• Synonyms
  • beta4Gal-T2
• GenBank acession
  • AF038660
• Locus
  1p34.1
• Discovery year
  1998-12-02
• Entrez gene record
  8704
• Pubmed identfication
  • 9405390
  • 9597550
• RefSeq identity
  • NM_003780
• Classification
  • Beta 4-glycosyltransferases
• VEGA ID
  OTTHUMG00000008296

Gene info

• Identity
  HGNC:24026
• Gene
  ACTRT2
• Long gene name
  actin related protein T2
• Synonyms
  • Arp-T2
  • ARPM2
  • FLJ25424
• GenBank acession
  • AF440740
  • AB057364
• Locus
  1p36.32
• Discovery year
  2005-11-22
• Entrez gene record
  140625
• Pubmed identfication
  • 11750065
  • 12243744
• RefSeq identity
  • NM_080431
• Classification
  • Actin related proteins
• VEGA ID
  OTTHUMG00000000562

Gene info

• Identity
  HGNC:10991
• Gene
  SLC25A5
• Long gene name
  solute carrier family 25 member 5
• Synonyms gene
  • ANT2
• Synonyms gene name
  • solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
• Synonyms
  • T2
  • 2F1
  • T3
• GenBank acession
  • BC068199
• Locus
  Xq24
• Discovery year
  1990-08-03
• Entrez gene record
  292
• Pubmed identfication
  • 2168878
  • 2829183
• RefSeq identity
  • NM_001152
• Classification
  • Solute carriers
• VEGA ID
  OTTHUMG00000022715
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:4836
• Gene
  HBZP1
• Long gene name
  hemoglobin subunit zeta pseudogene 1
• Synonyms gene
  • HBZP
• Synonyms gene name
  • hemoglobin, zeta pseudogene
  • hemoglobin, zeta pseudogene 1
• Synonyms
  • HBZ-T2
  • HBZ2
  • HBZ2P
• GenBank acession
  • J00184
• Locus
  16p13.3
• Discovery year
  2001-06-22
• Entrez gene record
  3051
• Pubmed identfication
  • 2649166
• VEGA ID
  OTTHUMG00000059925

Gene info

• Identity
  HGNC:15629
• Gene
  B3GNT2
• Long gene name
  UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
• Synonyms gene
  • B3GNT1
• Synonyms gene name
  • UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
• Synonyms
  • B3GNT-2
  • BETA3GNT
  • B3GN-T2
  • B3GN-T1
• GenBank acession
  • AB049584
• Locus
  2p15
• Discovery year
  2001-05-14
• Entrez gene record
  10678
• Pubmed identfication
  • 9892646
  • 11042166
• RefSeq identity
  • NM_006577
• Classification
  • MicroRNA protein coding host genes
  • Beta 3-glycosyltransferases
• VEGA ID
  OTTHUMG00000129444

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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