G418 Sulfate

Alternative_name

Antibiotic G418, G418
Description

Aminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
CAS Number

108321-42-2
Structure Available

Yes
Salt Form

Yes
Molecular Formula

C₂₀H₄₀N₄O₁₀. 2 H₂SO₄
Molecular Weight

692.71
Cell Permeable

No
Purity

>720 U/mg (potency)
Solubilities

H₂O (50 mg/ml)
Handling

Protect from light and moisture
Tag Line

An antibiotic and a selection agent
Storage Condition

-20°C
Shipping Condition

gel pack
Shelf Life

24months
MDL Number

MFCD00058314
PubChem CID

16760463
PubChem CID Link
SMILES

CC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O
InChi

InChI=1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)
InChi Key

UHEPSJJJMTWUCP-UHFFFAOYSA-N
Data Sheet Link
MSDS Available

Yes