G3BP Antibody

• Catalog number
  3619-30T
• Price
  Please ask
• Size
  30 µg

• Antibody Target
  G3BP
• Target Alternative Name
  ATP-dependent DNA helicase VIII
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide at C-terminal
• Accession
  Q13283
• Gene Id
  10146
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  30 µg (0.5 mg/ml) of antibody in PBS, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol, pH 7.2
• Purification
  Affinity purified
• Application
  Western blot
• Positive Control
  Western blot: Jurkat, 3T3, rat kidney cell lysate
• Application And Usages
  Western blot: 1:200
• Description
  G3BP1 (GTPase activating protein (SH3 domain) binding protein 1), also known as G3BP or HDH-VIII, is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. One of several DNA-unwinding enzymes, G3BP1 functions as a sequence-specific, phosphorylation-dependent helicase that unwinds partial RNA and DNA duplexes containing hanging 3’- or 5’- ends. G3BP1 uses magnesium as a cofactor and, in addition to its helicase activity, acts as an endoribonuclease that cleaves mRNA between adenine and cytosine residues at the 3’-UTR. An element of the Ras signaling pathway, G3BP1 binds to the SH3 domain of Ras GTPase-activating protein (Ras GAP) in proliferating cells, thereby regulating Ras signaling events in developing tissues. Due to its involvement in both DNA replication and signaling pathways within the cell, G3BP1 expression is implicated in the pathogenesis of several cancers, including esophageal squamous carcinoma.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  G3BP  
• Gene symbol
  G3BP1
• Short name
  G3BP Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  G3BP (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:30292
• Gene
  G3BP1
• Long gene name
  G3BP stress granule assembly factor 1
• Synonyms gene name
  • GTPase activating protein (SH3 domain) binding protein 1
• Synonyms
  • HDH-VIII
  • G3BP
• Synonyms name
  • Ras-GTPase-activating protein SH3-domain-binding protein
  • DNA helicase VIII
• GenBank acession
  • BC006997
• Locus
  5q33.1
• Discovery year
  2006-10-25
• Entrez gene record
  10146
• Pubmed identfication
  • 8649363
  • 9889278
  • 25229650
• RefSeq identity
  • NM_005754
• Classification
  • RNA binding motif containing
  • Endoribonucleases
• VEGA ID
  OTTHUMG00000130123

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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