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Description
A Competitive ELISA kit for in vitro quantitative determination of Florfenicol concentrations in Tissue, liver, Egg, Honey, Milk powder, Feed
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Detection Method
Absorbance (450 nm)
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Species Reactivity
Universal
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Applications
in vitro quantitative determination of Florfenicol concentrations in Tissue, liver, Egg, Honey, Milk powder, Feed
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Sample Type
Tissue, liver, Egg, Honey, Milk powder, Feed
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Features Benefits
Sensitivity: 0.15 ppb (ng/mL), Sample recovery rate: Tissue, Liver - 85%±20%; Honey- 85%±25%; Feed, Milk - 75%±25%., Cross-reactivity: Florfenicol -100%; Thiamphenicol - 0.1%, Detection range: Tissue, Liver, Honey, Milk - 0.075ppb; Feed, Milk powder - 0.15 ppb
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Storage Conditions
4°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Florfenicol is a broad-spectrum, primarily bacteriostatic, antibiotic with a range of activity similar to that of chloramphenicol, including many gram-negative and gram-positive organisms. It has a same mechanism of action as chloramphenicol i.e. inhibition of protein synthesis. Signs of Florfenicol toxicity are varied and include diarrhea and hyperbilirubinemia in horses, diarrhea and decreased feed consumption and rumen activity in cattle. Overdose or prolonged florfenicol administration can cause fatal bone marrow suppression. Florfenicol ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Florfenicol. During the reaction, Florfenicol in the samples or standard competes with Florfenicol coated on the plate for binding to the anti-Florfenicol antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Florfenicol. The concentration of Florfenicol in the samples can be calculated by comparing the OD of the samples to the standard curve.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Short name
Florfenicol ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Alternative name
Florfenicol Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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