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Alternative_name
1-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)-3-(4-hydroxyphenethyl)thiourea; Fluorescein isothiocyanate-tyramide
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Description
Tyramide signal amplification is an enzyme-catalyzed deposition of tyramide on or near a protein or nucleic acid in situ. The enzyme horseradish peroxidase (HRP) converts the labelled tyramide substrate into a highly reactive form that can covalently bind to tyrosine residues on target molecules at or near the HRP, in the presence of low concentrations of hydrogen peroxide. Labelled FITC-tyramide reagent enhances both the fluorescent and colorimetric detection sensitivity. FITC-tyramide is used in multiplex immunohostochemistry (IHC) and Fluorescent in situ hybridization (FISH). FITC-tyramide is useful for detecting marine picoplankton, in situ imaging of proteins and studying the effects of reactive oxygen species on extracellular matrix proteins.
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Structure Available
1
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Molecular Formula
C₂₉H₂₂N₂O₆S
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Molecular Weight
526.6
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Cell Permeable
1
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Purity
0.98
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Solubilities
~30 mg/ml in ethanol, ~35 mg/ml in DMSO
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Handling
Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
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Tag Line
A signal amplification reagent for IHC and FISH
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Storage Condition
-20ºC
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Shelf Life
36 months
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SMILES
S=C(NCCC1=CC=C(O)C=C1)NC2=CC3=C(C4(C5=C(OC6=C4C=CC(O)=C6)C=C(O)C=C5)OC3=O)C=C2
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InChi
InChI=1S/C29H22N2O6S/c32-18-4-1-16(2-5-18)11-12-30-28(38)31-17-3-8-22-21(13-17)27(35)37-29(22)23-9-6-19(33)14-25(23)36-26-15-20(34)7-10-24(26)29/h1-10,13-15,32-34H,11-12H2,(H2,30,31,38)
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InChi Key
IZHGETDBKCQWGN-UHFFFAOYSA-N
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MSDS Available
1
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Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
