EZScreen™ Lactate Dehydrogenase Activity Colorimetric Assay Kit (384-well)

• Catalog number
  K953-400
• Price
  Please ask
• Size
  400 assays

• Description
  Highly Sensitive, no sample preparation required
• Summary
  • Detection method- Absorbance (500 nm) • Species reactivity- Mammalian • Application- The kit can as low as 0.08 mU of LDH directly in samples.
• Detection Method
  Absorbance (500 nm)
• Species Reactivity
  Mammalians
• Applications
  • Measure triglycerides in biological samples • Screen and characterize compounds that influence triglyceride metabolism
• Sample Type
  Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.
• Features Benefits
  • Simple procedure; takes ~ 30 minutes • Fast and convenient • Sensitive assays for measuring LDH activity in various biological samples. The kit has been optimized for high throughput analyses
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Lactate dehydrogenase (LDH) is an oxidoreductase (EC 1.1.1.27) present in a wide variety of organisms. LDH catalyzes the interchange of lactate to pyruvate, with the concomitant interconversion of NAD+ to NADH. LDH is present in many organs and tissues including liver, heart, pancreas, kidneys, skeletal muscles, brain, and blood cells. When illness or injury damages cells, LDH may be released into the bloodstream, causing a rise of LDH level in blood. Elevated LDH in serum can indicate a number of unfavorable medical conditions like cerebral attack, heart attack, hepatitis, muscular dystrophy, hemolytic anemia and even certain cancers. Therefore, quantitation of LDH activity has significant clinical importance. BioVision’s EZScreenTM Lactate Dehydrogenase Colorimetric Assay kit, offers a simple and sensitive assay that can detect and quantify the enzymatic activity of LDH. In this assay, LDH oxidizes a substrate utilizing NAD as cofactor. The reduced cofactor (NADH) reacts with a chromophore producing a stable color (OD: 500 nm) that is directly proportional to LDH activity. The assay is suitable to detect the enzymatic activity as low as 0.08 mU in various biological samples
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  EZScreen™   Lactate   Dehydrogenase   Activity   Kit   384  
• Short name
  EZScreen™ Lactate Dehydrogenase Activity Colorimetric Assay Kit (384- )
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  EZScreen™ Lactate Dehydrogenase Activity colorimetry test reagent (384-well)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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