EZCell™ Thiol Detection Kit

• Catalog number
  K2063-100
• Price
  Please ask
• Size
  100 assays

• Description
  For detecting the intracellular thiol levels
• Summary
  BioVision’s EZCell™ Thiol Detection Kit utilizes a cell-permeable, thiol reactive fluorogenic Thiol Dye. The dye is strongly quenched and is essentially non-fluorescent until it reacts with an intracellular thiol moiety to generate a bright green fluorescence. The fluorescence can be quantified by measuring the fluorescence intensity in the FL1 channel (Ex/Em = 492/517 nm) of a flow cytometer. The thiol dye is selective for free reduced thiols, reacting with reduced GSH and cysteine to form highly fluorescent adducts, and does not label oxidized GSSG. The fluorescent conjugates are stable and are directly proportional to the intracellular thiol level. The EZCell™ Thiol Detection Kit provides a rapid, non-radioactive and sensitive method for measuring the overall intracellular thiol level in living cells and for studying the effects of oxidative stress and other thiol-modifying conditions.
• Detection Method
  Fluorescence (Ex/Em = 492/517 nm)
• Species Reactivity
  Mammalian
• Applications
  Detection of intracellular thiol level.,Assessment of drugs, which affect intracellular thiol level.
• Sample Type
  Adherent or Suspension cells
• Features Benefits
  EZCell™ Thiol Detection Kit provides a rapid, non-radioactive and sensitive method for measuring the overall intracellular thiol level in living cells.,The kit utilizes a cell-permeable, thiol reactive fluorogenic thiol dye that is selective for free reduced thiols.
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Thiols are organosulfur compounds that contain a free sulfhydryl group (-SH). The most predominant intracellular thiols in eukaryotes are cysteine, glutathione and cysteine-containing proteins. Thiols are vital in mediating cellular redox chemistry, preventing oxidative damage by reactive oxygen species and assisting in proper protein folding via the formation of disulfide bridges. Under physiological conditions, glutathione (GSH) is the most abundant intracellular thiol. High levels of reduced glutathione in the cytosol help maintain a reduced redox state, as GSH can react with the electrophilic xenobiotics and reduce protein thiols to support a variety of enzymatic redox reactions. Depletion of intracellular GSH and other thiols results in the buildup of lipid peroxidation products, indiscriminate oxidation of protein cysteine residues and is a strong activator of apoptosis. BioVision’s EZCell™ Thiol Detection Kit utilizes a cell-permeable, thiol reactive fluorogenic Thiol Dye. The dye is strongly quenched and is essentially non-fluorescent until it reacts with an intracellular thiol moiety to generate a bright green fluorescence. The fluorescence can be quantified by measuring the fluorescence intensity in the FL1 channel (Ex/Em = 492/517 nm) of a flow cytometer. The thiol dye is selective for free reduced thiols, reacting with reduced GSH and cysteine to form highly fluorescent adducts, and does not label oxidized GSSG. The fluorescent conjugates are stable and are directly proportional to the intracellular thiol level. The EZCell™ Thiol Detection Kit provides a rapid, non-radioactive and sensitive method for measuring the overall intracellular thiol level in living cells and for studying the effects of oxidative stress and other thiol-modifying conditions.
• Data Sheet Link
• Additional description
  The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

• Gene target
  EZCell™   Thiol   Kit  
• Short name
  EZCell™ Thiol Kit
• Technique
  detection
• Alternative name
  EZCell™ Thiol quantification reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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