EZCell™ Phagocytosis Assay Kit (Red E. coli)

• Catalog number
  K964-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Fluorescence (Ex/Em = 540/570 nm) • Applications- • Rapid detection, quantification and validation of phagocytosis
• Detection Method
  Fluorescence (Ex/Em = 540/570 nm)
• Species Reactivity
  Mammalian
• Applications
  Rapid detection, quantification and validation of phagocytosis in convenient 96-well format - Tracking ligand internalization and screening for effectors of phagocytosis
• Sample Type
  Phagocytic cell culture: adherent or suspension cells capable of phagocytosis
• Features Benefits
  • Simple & Rapid Protocol • Convenient: Non-Radioactive, no special handling or disposal required • High-Throughput • Accurate: reproducible results with low intra & inter assay variability
• Storage Conditions
  +4°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. E. coli and other bacterial strains are often used as a pathogen in phagocytosis assays. BioVision’s EZCell™ Phagocytosis Assay Kit (Red E. coli) utilizes heat-killed, fluorescently pre-labeled E. coli particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptors (TLR) ligands
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Conjugation
  red dye

• Gene target
  EZCell™   Phagocytosis   Kit   Red  
• Short name
  EZCell™ Phagocytosis Assay Kit (Red )
• Technique
  Assay, assays
• Host
  Assay, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
• Label
  Red
• Species
  E. coli
• Alternative name
  EZCell™ Phagocytosis test reagent (Red E. coli)
• Alternative technique
  arrays, escherichia, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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