EED (1- 441aa), Human recombinant

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• Catalog number
  7667-25
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• Size
  25 ug

# #

• Synonyms
  EED (1- 441aa), Human recombinant
• Alternative_names
  Embryonic Ectoderm Development
• Description
  Repressor of gene activity through histone deacetylation
• Recombinant
  Yes
• Source
  Insect (SF21) cells (baculovirus expression system)
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Molecular Weight
  53.5 kDa (1- 441 aa + NT His Tag)
• Storage Temp
  -80°C
• Shipping
  dry ice
• Shelf Life
  6 months
• Appearance
  Liquid
• Physical form description
  50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol.
• Background Information
  Polycomb protein Embryonic Ectoderm Development (EED) is a WD repeat containing member of the Polycomb-group (PcG) family. The EED protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED is an established transcriptional repressor, as a novel NIPP1 interactor. NIPP1 only interacted with full-length EED, whereas two EED interaction domains were mapped to the central and COOH-terminal thirds of NIPP1. In mammalian cells, EED is present as four distinct isoforms, which are believed to be produced by utilizing four distinct, in-frame translation start sites in a common EED mRNA. The extra sex combs (esc) gene of Drosophila and its mammalian homologue embryonic ectoderm development (EED) play pivotal roles in establishing Polycomb-group (Pc-G) mediated transcriptional silencing of regulatory genes during early development. EED/Sox2 regulatory loop contributes to the maintenance of self-renewal in embryonic stem (ES) cells by controlling histone methylation and acetylation. EED has been defined by the inability of embryos homozygous for certain c deletions to develop beyond the early stages of gastrulation.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

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• Gene target
  EED   441aa  
• Gene symbol
  EED
• Short name
  EED (1- 441aa), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  EED (1- 441aa), H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:3188
• Gene
  EED
• Long gene name
  embryonic ectoderm development
• Synonyms
  • WAIT-1
  • HEED
• Synonyms name
  • WD protein associating with integrin cytoplasmic tails 1
• GenBank acession
  • AF078933
• Locus
  11q14.2
• Discovery year
  1998-12-09
• Entrez gene record
  8726
• Pubmed identfication
  • 9765275
  • 9806832
• RefSeq identity
  • NM_003797
• Classification
  • WD repeat domain containing
  • MicroRNA protein coding host genes
  • Polycomb repressive complex 2
• VEGA ID
  OTTHUMG00000167209

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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