EED (1- 441aa), Human recombinant
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Catalog number7667-25
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Price:
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Size25 ug
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SynonymsEED (1- 441aa), Human recombinant
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Alternative_namesEmbryonic Ectoderm Development
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DescriptionRepressor of gene activity through histone deacetylation
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RecombinantYes
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SourceInsect (SF21) cells (baculovirus expression system)
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Molecular Weight53.5 kDa (1- 441 aa + NT His Tag)
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Storage Temp-80°C
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Shippingdry ice
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Shelf Life6 months
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AppearanceLiquid
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Physical form description50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol.
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Background InformationPolycomb protein Embryonic Ectoderm Development (EED) is a WD repeat containing member of the Polycomb-group (PcG) family. The EED protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED is an established transcriptional repressor, as a novel NIPP1 interactor. NIPP1 only interacted with full-length EED, whereas two EED interaction domains were mapped to the central and COOH-terminal thirds of NIPP1. In mammalian cells, EED is present as four distinct isoforms, which are believed to be produced by utilizing four distinct, in-frame translation start sites in a common EED mRNA. The extra sex combs (esc) gene of Drosophila and its mammalian homologue embryonic ectoderm development (EED) play pivotal roles in establishing Polycomb-group (Pc-G) mediated transcriptional silencing of regulatory genes during early development. EED/Sox2 regulatory loop contributes to the maintenance of self-renewal in embryonic stem (ES) cells by controlling histone methylation and acetylation. EED has been defined by the inability of embryos homozygous for certain c deletions to develop beyond the early stages of gastrulation.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolEED
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Short nameEED (1- 441aa), recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameEED (1- 441aa), H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameembryonic ectoderm development
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-12-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WD repeat domain containing
- MicroRNA protein coding host genes
- Polycomb repressive complex 2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data