DNA Polymerase I Large (Klenow) Fragment

Description

The large fragment of E. coli DNA Polymerase I. DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I
Product Highlights

1. DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs 2. cDNA second-strand synthesis 3. Generate single-stranded DNA probes using random primers 4. Site-directed DNA mutagenesis using synthetic oligonucleotides 5. Dideoxy DNA sequencing of single- or double-stranded DNA templates 6. 3’→5’ exonuclease activity can blunt a 3’-overhang
Storage Temp

-20°C
Shipping

gel pack
Shelf Life

12 months
Datasheet
Notes

For research use only. Not to be used for human or animal treatment or consumption.

Gene target

DNA Polymerase Klenow
Gene symbol

PRKDC, HLA-DOA
Short name

DNA Polymerase I (Klenow)
Technique

dna
Isotype

Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
Alternative name

Desoxyribonucleic acid polymerizing enzyme I large (Klenow) Fragment