DNA Bisulfite Conversion Kit II
-
Catalog number
K1479-200
-
Price
Please ask
-
Size
200 Preps
-
-
Description
Magnetic Beads based bisulfite DNA purification
-
Summary
Bisulfite modification of DNA and purification using spin columns
-
Detection Method
Bisulfite modification of DNA and purification using magnetic beads
-
Applications
An ideal tool for bisulfite modification of DNA and purification
-
Sample Type
Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. High-quality and RNA-free DNA is required.
-
Features Benefits
High recovery of DNA,High-throughput (96-well), bisulfite conversion of DNA in less than 2 h,Many downstream applications such as Next generation Sequencing, PCR amplification, etc.,Reliable and Ready-to-use
-
Storage Conditions
-20°C
-
Shipping Conditions
RT
-
Shelf life
12 months
-
Background
BioVision’s DNA Bisulfite Conversion Kit II is used for the bisulfite conversion of DNA that can be used to study the expression 5-methylcytosine. 5-methylcytosine is an epigenetic marker that plays an important role in differentiation, neurodegeneration, cancer etc. In this kit, DNA is treated with sodium bisulfite which leads to the deamination of cytosine residues and conversion to uracil, while 5-methyl cytosine residues remain unaffected. DNA Bisulfite Conversion Kit II integrates DNA denaturation and bisulfite conversion processes into a single step coupled to a magnetic bead based clean-up for high-throughput methylation analysis. The kit has been developed for high recovery of DNA following DNA bisulfite conversion. Desulphonation and clean-up of the converted DNA is performed while bound to the MagPure Beads. The kit is optimized to minimize template degradation, loss of DNA during treatment and clean-up and to provide complete conversion of unmethylated cytosine residues. The DNA recovered from this kit is used for downstream applications such as endonuclease digestion, Next Generation Sequencing, microarrays, PCR amplification, etc.
-
-
Gene target
-
Gene symbol
PRKDC, HLA-DOA
-
Short name
DNA Bisulfite Conversion Kit II
-
Technique
dna
-
Alternative name
Desoxyribonucleic acid Bisulfite Conversion reagent II
-
Alternative technique
kits
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-
Gene info
Gene info
Product images
Similar products
