Cytosol/Particulate Separation Kit

• Catalog number
  K267-50
• Price
  Please ask
• Size
  50 assays

• Description
  Rapidly separates cytosol from particulate fractions
• Summary
  • Species reactivity- Mammalian • Applications- The Cytosol/Particulate Separation Kit provides an easy and convenient system for a rapid separation of cytosol and particulate fractions. Assaying cellular distributions of small moleculars or proteins, and other signal transduction pathways
• Sample Type
  Cell cultures (adherent and suspension), fresh and frozen tissues
• Features Benefits
  • Simple procedure; takes less than 1 hour • Fast and convenient • The new Cytosol/Particulate Rapid Separation Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components. However, traditional methods usually take hours to separate cytosol from particulates, so that some cellular components especially small molecules and metabolites are getting diffused or redistributed during the separation procedure. The new Cytosol/Particulate Rapid Separation Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules. Subcellular localization and analyses of factors interested can be performed accurately.
• Data Sheet Link

• Gene target
  Cytosol/Particulate   Separation   Kit  
• Short name
  Cytosol/Particulate Separation Kit
• Technique
  separation
• Alternative name
  cytoplasm/Particulate Separation reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products