CNTF, human recombinant
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Catalog number4017-1000
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Price:
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Size1 mg
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SynonymsHCNTF, CNTF, Ciliary Neurotrophic Factor.
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Alternative_namesHCNTF, CNTF, Ciliary Neurotrophic Factor.
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DescriptionA potent neural factor promoting survivability and differentiation of neuronal cell types.
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Biological activityThe ED₅₀ as determined by the dose-dependent proliferation of human TF-1 cells is < 2 ng/ml, corresponding to a specific activity of > 5 x 10⁵units/mg.
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Results< 2 ng/ml
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Molecular Weight22.9 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionSterile filtered and lyophilized without additives
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Reconstitution InstructionsReconstitute in 5 mM Sodium Phosphate, pH 7.5 to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use
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Background InformationCNTF (Ciliary Neurotrophic Factor) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. CNTF has also been shown to promote survivability and differentiation of other neuronal cell types. Recombinant human CNTF is a 22.9 kDa protein containing 200 amino acid residues
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolZFP91-CNTF, CNTF
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Short nameCNTF, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameciliary neurotrophic factor, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetciliary neurotrophic factor, HCNTF, CNTF and IDBG-409278 and ENSG00000242689 and 1270, growth factor activity, Extracellular, Cntf and IDBG-254935 and ENSMUSG00000079415 and 12803, CNTF and IDBG-637978 and ENSBTAG00000003624 and 100848228
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Gene info
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Identity
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Gene
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Long gene nameZFP91-CNTF readthrough (NMD candidate)
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GenBank acession
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Locus
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Discovery year2011-05-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameciliary neurotrophic factor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-01-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukin 6 type cytokine family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data