CD45 FITC Monoclonal Antibody (Clone HI30)
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Catalog number6958-25
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Price:
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Size25 tests
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Antibody TargetCD45
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Target Alternative NameCD45
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Antibody TypeMonoclonal
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CloneHI30
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Species ReactivitiesHuman
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Immunogen SequenceHuman CD45
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AccessionP08575
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Gene Id5788
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AppearanceColorless liquid
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FormLiquid
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FormulationPhosphate-buffered aqueous solution pH 7.2, ≤0.09% Sodium azide, may contain carrier protein/stabilizer.
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PurificationAffinity chromatography.
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ApplicationFlow cytometry
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Positive ControlFACS: Human peripheral blood lymphocytes
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Application And UsagesFlow (Cell Surface): 5 µl/1x10^6 cells, Volume per test: 5 µl (0.25 µg).
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DescriptionCD45, a transmembrane multifunctional glycoprotein, is a member of Type I receptor-linked PTPase family and is expressed as multiple isoforms due to alternative splicing. Expression of these isoforms is highly regulated and shift in this expression determines T-cell activation. CD45RB consists of exon B and is predominantly expressed in naïve T-cells secreting IL-2. Its expression is low in primed/memory T cells, cells that express Th2 cytokines such as IL-4 and IL-10 and population of T-cells with regulatory function. Immunotherapy with CD45RB antibody is being widely studied in transplantation and vaccination. CD45 antibodies are commonly used to identify tumors of lymphoid origin. The HI130 monoclonal antibody specifically reacts with the 180 kDa, 190 kDa, 205 kDa, and 220 kDa isoforms of the human leukocyte common antigen (LCA) CD45. It is expressed on lymphocytes, granulocytes, monocytes, thymocytes, and eosinophils, but not on mature erythrocytes, platelets, mature erythroid cells of bone marrow, and non-hematopoietic tissues. CD45 is essential for T cell activation and the tyrosine phosphatase activity of its intracellular region is integral for signal transduction.
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Storage Condition4°C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedNot Verified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedVerified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolPTPRCAP, PTPRC, AMBP
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Short nameCD45 FITC Monoclonal Antibody (Clone HI30)
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
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IsotypeMouse IgG1, Kappa
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LabelFITC
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Alternative nameCD45 fluorecein monoclonal (antibody to-) (clonality HI30)
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Alternative techniqueantibodies, fluorescine
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Clone nameHI30
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Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C associated protein
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Synonyms
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Locus
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Discovery year1996-04-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namealpha-1-microglobulin/bikunin precursor
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Lipocalins
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VEGA ID