CD40Ligand/TRAP, murine recombinant
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Catalog number4015-1000
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Price:
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Size1 mg
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SynonymsCD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L.
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Alternative_namesCD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L.
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DescriptionA protein primarily expressed on activated T cells and involved in immune functions
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Biological activityDetermined by its ability to induce TNF-α and MIP-1α production by murine splenocytes. The expected ED₅₀ for this effect is
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Molecular Weight16.4 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionSterile filtered and then lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 0.1 M Arginine.
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Reconstitution InstructionsReconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.
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Background InformationCD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant murine CD40 Ligand is a soluble 18 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolACP5, TGFBRAP1, CD40LG, SCYL1
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Short nameCD40Ligand/TRAP, murine recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative nameCD40Ligand/TRAP, murine Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameacid phosphatase 5, tartrate resistant
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-02-23
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Entrez gene record
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Pubmed identfication
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Classification
- Acid phosphatases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nametransforming growth factor beta receptor associated protein 1
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Synonyms gene name
- transforming growth factor, beta receptor associated protein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CORVET complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD40 ligand
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Tumor necrosis factor superfamily
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameSCY1 like pseudokinase 1
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Synonyms gene
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Synonyms gene name
- N-terminal kinase-like
- SCY1-like 1 (S. cerevisiae)
- SCY1-like, kinase-like 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-01-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
- SCY1 like pseudokinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data