Cathepsin D Activity Fluorometric Assay Kit
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Catalog numberK143-100
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Price:
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Size100 assays
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Summary• Detection method- Fluorescence (Ex/Em 328/460) • Species reactivity- Mammalian • Kit size- 100 assays • Applications- Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.
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Sample TypeCell and tissue lysates
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Features Benefits• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • Cathepsin-D assay is straightforward, and can be adapted to 96-well plate assays.
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life6-12 months
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BackgroundApoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with MCA. Cell lysates or other samples that contain cathepsin-D will cleave the synthetic substrate to release fluorescence, which can then easily be quantified using a fluorometer or fluorescence plate reader at Ex/Em = 328/460 nm.
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TestBiovision supplies other types of Assays as 1.
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GeneCTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
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Gene target
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Short nameCathepsin D Activity Fluorometric Assay Kit
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TechniqueAssay, assays
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HostAssay
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Alternative nameCathepsin D Activity fluorescence test reagent
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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