CaspGLOW™ Red Active Caspase Staining Kit
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Catalog numberK190-100
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Price:
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Size100 assays
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DescriptionDetects activated Caspases by FACS, FL or plate reader
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Summary• Detection method- Fluorescence microscopy, fluorescence plate reader (Ex/Em = 540/570 nm) and Flow cytometry • Species reactivity- Mammalian • Kit size- Convenient sizes (25 and 100 assays) • Applications- Detection of activated caspases in living cells.
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Sample TypeLive cells
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Features Benefits• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The red fluorescence label allows for direct detection of activated caspases in apoptotic cells
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life6-12 months
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BackgroundActivation of caspases plays a central role in apoptosis. The CaspGLOWTM Red Active Caspase Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes a caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.
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Additional descriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Conjugationred dye
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Gene target
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Short nameCaspGLOW™ Red Active Caspase Staining Kit
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LabelRed
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Alternative nameCaspGLOW™ Red functionnal caspase coloring reagent
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Alternative techniquekits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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ConceptScope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
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