Caspase-3, mouse recombinant
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Catalog number1183-25
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Price:
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Size25 units
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SynonymsCaspase 3
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Alternative_namesCaspase-3, Short name=CASP-3, Apopain, Cysteine protease CPP32, Short name=CPP-32, FLICE, Protein Yama, SREBP cleavage activity 1, Short name=SCA-1
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DescriptionA cysteine-aspartic acid protease involved in apoptosis
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RecombinantYes
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SourceE. coli
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AssaySDS-PAGE
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Activity Specifications test method300000 units/mg
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Unit DefinitionOne unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Molecular Weightof large (17 kD) and small (11 kD) subunits
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Storage Temp-70°C
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Shippinggel pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionLyophilized powder
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Reconstitution InstructionsReconstitute to 1 unit per µl in PBS containing 15% glycerol
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Background InformationCaspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional descriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCASP1P3, CASP3, CASP3P1
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Short nameCaspase-3, mouse recombinant
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Hostmouse
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SpeciesMouse, Mouses
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Alternative namecaspase-3, mouse Rec.
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Alternative techniquerec, murine
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Gene info
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Identity
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Gene
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Long gene namecaspase 1 pseudogene 3
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Synonyms gene name
- caspase 1, apoptosis-related cysteine peptidase pseudogene 3
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Locus
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Discovery year2012-02-24
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namecaspase 3
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Synonyms gene name
- caspase 3, apoptosis-related cysteine protease
- caspase 3, apoptosis-related cysteine peptidase
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-07-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Small nucleolar RNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecaspase 3 pseudogene 1
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Synonyms gene name
- caspase 3, apoptosis-related cysteine peptidase pseudogene 1
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Locus
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Discovery year2011-11-22
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
- E05.599.495