Caspase-13 Substrate LEED-AFC
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Catalog number1116-200
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PricePlease ask
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Size200 assays
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Alternative_nameAc-LEED-AFC, Caspase-13 Substrate, Fluorogenic
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DescriptionReady-to-use fluorometric substrate for caspase-13 and related caspases that recognize the amino acid sequence LEED. Caspase-13 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEED-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
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Structure AvailableNo
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Peptide sequenceAc-Leu-Glu-Glu-Asp-AFC
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Salt FormNo
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Molecular FormulaC₃₂H₃₈F₃N₅O₁₃
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Molecular Weight757.66
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Cell PermeableNo
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Purity>98% by HPLC
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HandlingProtect from light and moisture
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Tag LineA caspase-13 substrate
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Storage Condition-20°C
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Shipping Conditiongel pack
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Shelf Life12 months
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MDL NumberMFCD03452816
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PubChem CID44135106
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SMILESCC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C
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InChiInChI=1S/C32H38F3N5O13/c1-14(2)10-21(36-15(3)41)31(52)39-19(6-8-24(42)43)28(49)38-20(7-9-25(44)45)29(50)40-22(13-26(46)47)30(51)37-16-4-5-17-18(32(33,34)35)12-27(48)53-23(17)11-16/h4-5,11-12,14,19-22H,6-10,13H2,1-3H3,(H,36,41)(H,37,51)(H,38,49)(H,39,52)(H,40,50)(H,42,43)(H,44,45)(H,46,47)
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InChi KeyZPNCQMJLTSFJQH-UHFFFAOYSA-N
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MSDS AvailableYes
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Additional descriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Gene target
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Short nameCaspase-13 Substrate LEED-AFC
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Alternative namecaspase-13 Substrate LEED-AFC
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MeSH Data
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Name
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ConceptScope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
- E05.599.495