Caspase-10/a, human recombinant
#
-
Catalog number1090A-100
-
Price:
-
Size100 units
-
-
SynonymsCaspase-10/a
-
Alternative_namesCaspase-10, CASP-10, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE2, ICE-like apoptotic protease 4
-
DescriptionA cysteine protease involved in apoptosis
-
RecombinantYes
-
SourceE. coli.
-
Purity by SDS PAGE≥90%
-
AssaySDS-PAGE
-
Activity Specifications test method> 8000 units/mg
-
Unit DefinitionOne unit of the recombinant caspase-10/a is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
-
Molecular Weighttwo large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form.
-
Storage Temp-70°C
-
Shippinggel pack
-
Shelf Life12 months
-
AppearanceLyophilized powder
-
Physical form descriptionLyophilized powder
-
Reconstitution InstructionsReconstitute to 1 unit per ul in PBS containing 15% glycerol.
-
Background InformationCaspase-10/a (also known as Mch4) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form. The recombinant active human caspase-10 was expressed in E. coli. The active caspase-10 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
Additional descriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional sourceRecombinants or rec. proteins
-
Grouprecombinants
-
Gene target
-
Gene symbolMINDY3
-
Short nameCaspase-10/a, recombinant
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
SpeciesHuman, Humans
-
Alternative namecaspase-10/a, H. sapiens Rec.
-
Alternative techniquerec
-
Gene info
-
Identity
-
Gene
-
Long gene nameMINDY lysine 48 deubiquitinase 3
-
Synonyms gene
-
Synonyms gene name
- chromosome 10 open reading frame 97
- family with sequence similarity 188 member A
- MINDY deubiquitinase 3
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2004-04-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- MINDY deubiquitinases
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
-
Tree numbers
- E05.599.495