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Description
High-throughput compatible, quick and easy screening for Carbonic Anhydrase ligands; Includes human recombinant NEP
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Summary
• Detection method - Colorimetric (OD 405 nm) • Species reactivity - N/A • Applications - • Screening of drugs and novel ligands for interaction with human recombinant Carbonic Anhydrase
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Detection Method
Detection method - Colorimetric (OD 405 nm)
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Species Reactivity
N/A
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Applications
Screening of drugs and novel ligands for interaction with human recombinant Carbonic Anhydrase
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Sample Type
Samples containing drugs, inhibitors or ligands (compounds that can interact and affect CA activity)
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Features Benefits
• Simple, highly sensitive, high-throughput compatible • Rapid screening of Carbonic Anhydrase ligands • Kit includes Acetazolamide, a CA inhibitor, and a stable, recombinant human CA
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Carbonic anhydrases (CA; EC: 4.2.1.1) are zinc enzymes present in both prokaryotes and eukaryotes. They efficiently catalyze the reversible hydration of CO2 to bicarbonate. Their important patho-physiological roles in respiration, pH and CO2 homeostasis, secretion, gluconeogenesis, ureagenesis etc. make it an important drug target. CA inhibition in different organs can result in different effects. Inhibition of CA helps in different disease treatment procedures; for example, it can help in the treatment of Glaucoma, urine alkalization, managing epilepsy, sleep apnea, acute mountain sickness etc. BioVision’s CA Inhibitor Screening Kit can be used to screen for potent inhibitors of CA activity. It utilizes the esterase activity of an active CA on an ester substrate which releases a chromogenic product. The released product can be easily quantified using an absorbance microplate reader. In the presence of a CA specific inhibitor, the enzyme loses its activity which results in decrease of absorbance. This assay kit is simple and can be used to identify and characterize CA inhibitors in a high-throughput format. Here, we have used the specific inhibitor Acetazolamide, a potent inhibitor of CA I, CA II, CA IV, CA IX, CA XII etc. except CA III
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
