BRG1 Antibody
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Catalog number3739-100
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PricePlease ask
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Size100 µl
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Antibody TargetBRG1
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Target Alternative NameBRG1, BRM/SWI2-Related Gene 1, SMARCA4, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, member 4, SNF2L4, Sucrose Nonfermenting 2-Like 4
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse
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Immunogen SequenceSynthetic peptide from a portion of human BRG1/SMARCA4 protein
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AccessionP51532
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Gene Id6597
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µl purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
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PurificationAffinity purified
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ApplicationWestern Blot
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Application And UsagesWestern blotting (1:500 – 1:2500 µg/ml). However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control. The antibody recognizes the BRG1/SMARCA4 of human and mouse origins. Reactivity to other species has not been tested.
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DescriptionThe acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRG1 is a member of the SWI/SNF protein family, which forms part of a large ATP-dependent chromatin remodeling complex. This complex is required for transcriptional activation of genes normally repressed by chromatin. BRG1 is mutated in many cancer cell lines, such as breast, prostate, lung, pancreas and colon. Further, BRG1 has an important role as a tumor suppressor. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSMARCA4
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Short nameBRG1 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameBRG1 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Bromodomain containing
- PBAF complex
- BAF complex
- GBAF complex
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data