BioSim™ anti-Cetuximab II (Human) ELISA Kit
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Catalog number
E4873-100
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Price
Please ask
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Size
96 assays
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Description
A Sandwich ELISA kit for the qualitative measurement of antibodies to Cetuximab in human serum or plasma samples
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Detection Method
Absorbance (450 nm)
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Species Reactivity
Human
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Applications
This Sandwich ELISA kit is used for in vitro qualitative determination of antibodies to Cetuximab in human serum and plasma samples
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Sample Type
Plasma,Serum
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Features Benefits
Cross Reactivity: Except for Cetuximab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins,Assay Precision: Intra-Assay and Inter-Assay CV < 30%,For in vitro qualitative determination of antibodies to Cetuximab in human serum and plasma samples
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Storage Conditions
4ºC
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Cetuximab is a recombinant human/mouse chimeric monoclonal antibody that targets the epidermal growth factor receptor (EGFR) and acts as a competitive inhibitor for both epidermal growth factor and other ligands. Binding of the antibody to EGFR causes dimerization of the receptor and endocytosis which results in cell-cycle arrest and cell death. The monoclonal antibody is used to treat several forms of cancer such as metastatic colorectal cancer, non-small cell lung cancer, head and neck cancer. BioSim™ anti-Cetuximab ELISA kit has been specifically developed to qualitatively determine antibodies to Cetuximab in human serum and plasma samples. The ELISA kit is based on the sandwich principle. Controls and samples (serum and plasma) are incubated in the microtiter plate coated with the drug Cetuximab. After incubation, the wells are washed and the enzymatic activity is detected by the addition of chromogen-substrate. The enzyme-substrate reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of antibodies specific for Cetuximab present in the samples and controls. The qualitative test results can be evaluated using cut-off value.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Short name
BioSim™ anti-Cetuximab II ( ) ELISA Kit
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Technique
ELISA kit, anti-, ELISA, anti, antibody to, antibodies, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Humans
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Alternative name
BioSim™ Antibody toCetuximab II (H. sapiens) Enzyme-linked immunosorbent assay reagent
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Alternative technique
antibodies, elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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